2hbs: Difference between revisions

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-[[Image:2hbs.gif|left|200px]]
+[[Image:2hbs.png|left|200px]]
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-==Overview==
+{{ABSTRACT_9325099}}
-We have refined the crystal structure of deoxyhemoglobin S (beta Glu6--&gt;Val) at 2.05 A resolution to an R-factor of 16.5% (free R=21. 5%) using crystals isomorphous to those originally grown by Wishner and Love. A predominant feature of this crystal form is a double strand of hemoglobin tetramers that has been shown by a variety of techniques to be the fundamental building block of the intracellular sickle cell fiber. The double strand is stabilized by lateral contacts involving the mutant valine interacting with a pocket between the E and F helices on another tetramer. The new structure reveals some marked differences from the previously refined 3.0 A resolution structure, including several residues in the lateral contact which have shifted by as much as 3.5 A. The lateral contact includes, in addition to the hydrophobic interactions involving the mutant valine, hydrophilic interactions and bridging water molecules at the periphery of the contact. This structure provides further insights into hemoglobin polymerization and may be useful for the structure-based design of therapeutic agents to treat sickle cell disease.
 ==About this Structure==
 HBS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 2HBS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb41_1.html Hemoglobin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HBS OCA].
-==Reference==
-The high resolution crystal structure of deoxyhemoglobin S., Harrington DJ, Adachi K, Royer WE Jr, J Mol Biol. 1997 Sep 26;272(3):398-407. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9325099 9325099]
 [[Category: Hemoglobin]]
 [[Category: Homo sapiens]]
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 [[Category: Hemoglobin]]
 [[Category: Oxygen transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon May 26 21:55:23 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:17:35 2008''