1hwg: Difference between revisions

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[[Image:1hwg.gif|left|200px]]
[[Image:1hwg.png|left|200px]]


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==Overview==
{{ABSTRACT_8943276}}
Human growth hormone binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, growth hormone acts as an antagonist because of a large difference in affinities at the respective binding sites. This antagonist action can be enhanced further by reducing binding in the low affinity binding site. A growth hormone antagonist mutant Gly-120 --&gt; Arg, has been crystallized with its receptor as a 1:1 complex and the crystal structure determined at 2.9 A resolution. The 1:1 complex is remarkably similar to the native growth hormone-receptor 1:2 complex. A comparison between the two structures reveals only minimal differences in the conformations of the hormone or its receptor in the two complexes, including the angle between the two immunoglobulin-like domains of the receptor. Further, two symmetry-related 1:1 complexes in the crystal form a 2:2 complex with a large solvent inaccessible area between two receptor molecules. In addition, we present here a native human growth hormone-human growth hormone-binding protein 1:2 complex structure at 2.5 A resolution. One important difference between our structure and the previously published crystal structure at 2.8 A is revealed. Trp-104 in the receptor, a key residue in the hormone-receptor interaction, has an altered conformation in the low affinity site enabling a favorable hydrogen bond to be formed with Asp-116 of the hormone.


==About this Structure==
==About this Structure==
1HWG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1HWG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb52_1.html Growth Hormone]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWG OCA].  
1HWG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1HWG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb52_1.html Growth Hormone]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWG OCA].  
==Reference==
Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 A resolution., Sundstrom M, Lundqvist T, Rodin J, Giebel LB, Milligan D, Norstedt G, J Biol Chem. 1996 Dec 13;271(50):32197-203. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8943276 8943276]
[[Category: Growth Hormone]]
[[Category: Growth Hormone]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Hormone]]
[[Category: Hormone]]
[[Category: Receptor]]
[[Category: Receptor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:17:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:30:39 2008''

Revision as of 17:30, 26 June 2008

File:1hwg.png

Template:STRUCTURE 1hwg

1:2 COMPLEX OF HUMAN GROWTH HORMONE WITH ITS SOLUBLE BINDING PROTEIN


Template:ABSTRACT 8943276

About this StructureAbout this Structure

1HWG is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1HWG with [Growth Hormone]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:30:39 2008

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