1kw8: Difference between revisions

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[[Image:1kw8.jpg|left|200px]]
[[Image:1kw8.png|left|200px]]


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==Overview==
{{ABSTRACT_12206778}}
BphC derived from Pseudomonas sp. strain KKS102 is an extradiol-cleaving catecholic dioxygenase. This enzyme contains a non-heme iron atom and plays an important role in degrading biphenyl/polychlorinated biphenyls (PCBs) in the microbe. To elucidate detailed structures of BphC reaction intermediates, crystal structures of the substrate-free form, the BphC-substrate complex, and the BphC-substrate-NO (nitric oxide) complex were determined. These crystal structures revealed (1) the binding site of the O(2) molecule in the coordination sphere and (2) conformational changes of His194 during the catalytic reaction. On the basis of these findings, we propose a catalytic mechanism for the extradiol-cleaving catecholic dioxygenase in which His194 seems to play three distinct roles. At the early stage of the catalytic reaction, His194 appears to act as a catalytic base, which likely deprotonates the hydroxyl group of the substrate. At the next stage, the protonated His194 seems to stabilize a negative charge on the O2 molecule located in the hydrophobic O2-binding cavity. Finally, protonated His194 seems to function as a proton donor, whose existence has been proposed.


==About this Structure==
==About this Structure==
1KW8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1gdg 1gdg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KW8 OCA].  
1KW8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1gdg 1gdg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KW8 OCA].  
==Reference==
Crystal structures of the reaction intermediate and its homologue of an extradiol-cleaving catecholic dioxygenase., Sato N, Uragami Y, Nishizaki T, Takahashi Y, Sazaki G, Sugimoto K, Nonaka T, Masai E, Fukuda M, Senda T, J Mol Biol. 2002 Aug 23;321(4):621-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12206778 12206778]
[[Category: Biphenyl-2,3-diol 1,2-dioxygenase]]
[[Category: Biphenyl-2,3-diol 1,2-dioxygenase]]
[[Category: Pseudomonas sp.]]
[[Category: Pseudomonas sp.]]
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[[Category: Uragami, Y.]]
[[Category: Uragami, Y.]]
[[Category: Four time repetitions of the beta-alpha-beta-beta-beta motif]]
[[Category: Four time repetitions of the beta-alpha-beta-beta-beta motif]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:14:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 19:17:49 2008''

Revision as of 19:17, 25 June 2008

File:1kw8.png

Template:STRUCTURE 1kw8

Crystal structure of BphC-2,3-dihydroxybiphenyl-NO complex


Template:ABSTRACT 12206778

About this StructureAbout this Structure

1KW8 is a Single protein structure of sequence from Pseudomonas sp.. This structure supersedes the now removed PDB entry 1gdg. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 19:17:49 2008

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