1gjh: Difference between revisions

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[[Image:1gjh.gif|left|200px]]
[[Image:1gjh.png|left|200px]]


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==Overview==
{{ABSTRACT_11248023}}
The structures of two isoforms of Bcl-2 that differ by two amino acids have been determined by NMR spectroscopy. Because wild-type Bcl-2 behaved poorly in solution, the structures were determined by using Bcl-2/Bcl-x(L) chimeras in which part of the putative unstructured loop of Bcl-2 was replaced with a shortened loop from Bcl-x(L). These chimeric proteins have a low pI compared with the wild-type protein and are soluble. The structures of the two Bcl-2 isoforms consist of 6 alpha-helices with a hydrophobic groove on the surface similar to that observed for the homologous protein, Bcl-x(L). Comparison of the Bcl-2 structures to that of Bcl-x(L) shows that although the overall fold is the same, there are differences in the structural topology and electrostatic potential of the binding groove. Although the structures of the two isoforms of Bcl-2 are virtually identical, differences were observed in the ability of the proteins to bind to a 25-residue peptide from the proapoptotic Bad protein and a 16-residue peptide from the proapoptotic Bak protein. These results suggest that there are subtle differences in the hydrophobic binding groove in Bcl-2 that may translate into differences in antiapoptotic activity for the two isoforms.


==About this Structure==
==About this Structure==
1GJH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1g5o 1g5o]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJH OCA].  
1GJH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1g5o 1g5o]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJH OCA].  
==Reference==
Solution structure of the antiapoptotic protein bcl-2., Petros AM, Medek A, Nettesheim DG, Kim DH, Yoon HS, Swift K, Matayoshi ED, Oltersdorf T, Fesik SW, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3012-7. Epub 2001 Feb 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11248023 11248023]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Yoon, H S.]]
[[Category: Yoon, H S.]]
[[Category: Apoptosis]]
[[Category: Apoptosis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:39:12 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 19:17:40 2008''

Revision as of 19:17, 25 June 2008

File:1gjh.png

Template:STRUCTURE 1gjh

HUMAN BCL-2, ISOFORM 2


Template:ABSTRACT 11248023

About this StructureAbout this Structure

1GJH is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1g5o. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 19:17:40 2008

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