2zjp: Difference between revisions
New page: '''Unreleased structure''' The entry 2zjp is ON HOLD Authors: Harms, J.M., Wilson, D.N., Schluenzen, F., Connell, S.R., Stachelhaus, T., Zaborowska, Z., Spahn, C.M., Fucini, P. Descrip... |
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[[Image:2zjp.jpg|left|200px]] | |||
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{{STRUCTURE_2zjp| PDB=2zjp | SCENE= }} | |||
'''Thiopeptide antibiotic Nosiheptide bound to the large ribosomal subunit of Deinococcus radiodurans''' | |||
==Overview== | |||
The thiopeptide class of antibiotics targets the GTPase-associated center (GAC) of the ribosome to inhibit translation factor function. Using X-ray crystallography, we have determined the binding sites of thiostrepton (Thio), nosiheptide (Nosi), and micrococcin (Micro), on the Deinococcus radiodurans large ribosomal subunit. The thiopeptides, by binding within a cleft located between the ribosomal protein L11 and helices 43 and 44 of the 23S rRNA, overlap with the position of domain V of EF-G, thus explaining how this class of drugs perturbs translation factor binding to the ribosome. The presence of Micro leads to additional density for the C-terminal domain (CTD) of L7, adjacent to and interacting with L11. The results suggest that L11 acts as a molecular switch to control L7 binding and plays a pivotal role in positioning one L7-CTD monomer on the G' subdomain of EF-G to regulate EF-G turnover during protein synthesis. | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | ==About this Structure== | ||
2ZJP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJP OCA]. | |||
==Reference== | |||
Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin., Harms JM, Wilson DN, Schluenzen F, Connell SR, Stachelhaus T, Zaborowska Z, Spahn CM, Fucini P, Mol Cell. 2008 Apr 11;30(1):26-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18406324 18406324] | |||
[[Category: Deinococcus radiodurans]] | |||
[[Category: Protein complex]] | |||
[[Category: Connell, S R.]] | |||
[[Category: Fucini, P.]] | |||
[[Category: Harms, J M.]] | |||
[[Category: Schluenzen, F.]] | |||
[[Category: Spahn, C M.T.]] | |||
[[Category: Stachelhaus, T.]] | |||
[[Category: Wilson, D N.]] | |||
[[Category: Zaborowska, Z.]] | |||
[[Category: 50]] | |||
[[Category: Complex]] | |||
[[Category: L11]] | |||
[[Category: Metal-binding]] | |||
[[Category: Methylation]] | |||
[[Category: Molecular switch]] | |||
[[Category: Ribonucleoprotein]] | |||
[[Category: Ribosomal protein]] | |||
[[Category: Ribosomal subunit]] | |||
[[Category: Ribosome]] | |||
[[Category: Rna-binding]] | |||
[[Category: Rrna-binding]] | |||
[[Category: Thiopeptide antibiotic]] | |||
[[Category: Translational regulation]] | |||
[[Category: Trna-binding]] | |||
[[Category: Zinc]] | |||
[[Category: Zinc-finger]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:07:36 2008'' | |||