2vpd: Difference between revisions
New page: '''Unreleased structure''' The entry 2vpd is ON HOLD until Paper Publication Authors: Fiedler, M., Sanchez-Barrena, M.J., Nekrasov, M., Mieszczanek, J., Rybin, V., Muller, J., Bienz, M.... |
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[[Image:2vpd.jpg|left|200px]] | |||
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{{STRUCTURE_2vpd| PDB=2vpd | SCENE= }} | |||
'''DECODING OF METHYLATED HISTONE H3 TAIL BY THE PYGO-BCL9 WNT SIGNALING COMPLEX''' | |||
==Overview== | |||
Pygo and BCL9/Legless transduce the Wnt signal by promoting the transcriptional activity of beta-catenin/Armadillo in normal and malignant cells. We show that human and Drosophila Pygo PHD fingers associate with their cognate HD1 domains from BCL9/Legless to bind specifically to the histone H3 tail methylated at lysine 4 (H3K4me). The crystal structures of ternary complexes between PHD, HD1, and two different H3K4me peptides reveal a unique mode of histone tail recognition: efficient histone binding requires HD1 association, and the PHD-HD1 complex binds preferentially to H3K4me2 while displaying insensitivity to methylation of H3R2. Therefore, this is a prime example of histone tail binding by a PHD finger (of Pygo) being modulated by a cofactor (BCL9/Legless). Rescue experiments in Drosophila indicate that Wnt signaling outputs depend on histone decoding. The specificity of this process provided by the Pygo-BCL9/Legless complex suggests that this complex facilitates an early step in the transition from gene silence to Wnt-induced transcription. | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | ==About this Structure== | ||
2VPD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPD OCA]. | |||
==Reference== | |||
Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex., Fiedler M, Sanchez-Barrena MJ, Nekrasov M, Mieszczanek J, Rybin V, Muller J, Evans P, Bienz M, Mol Cell. 2008 May 23;30(4):507-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18498752 18498752] | |||
[[Category: Homo sapiens]] | |||
[[Category: Protein complex]] | |||
[[Category: Bienz, M.]] | |||
[[Category: Evans, P.]] | |||
[[Category: Fiedler, M.]] | |||
[[Category: Mieszczanek, J.]] | |||
[[Category: Muller, J.]] | |||
[[Category: Nekrasov, M.]] | |||
[[Category: Rybin, V.]] | |||
[[Category: Sanchez-Barrena, M J.]] | |||
[[Category: Bcl9 hd1 domain]] | |||
[[Category: Chromosomal rearrangement]] | |||
[[Category: Gene regulation]] | |||
[[Category: Histone h3k4me2]] | |||
[[Category: Hpygo1 phd domain]] | |||
[[Category: Metal-binding]] | |||
[[Category: Nucleus]] | |||
[[Category: Phosphoprotein]] | |||
[[Category: Proto-oncogene]] | |||
[[Category: Signaling protein]] | |||
[[Category: Wnt signaling complex]] | |||
[[Category: Wnt signaling pathway]] | |||
[[Category: Zinc]] | |||
[[Category: Zinc-finger]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:05:27 2008'' | |||
Revision as of 12:05, 18 June 2008
DECODING OF METHYLATED HISTONE H3 TAIL BY THE PYGO-BCL9 WNT SIGNALING COMPLEX
OverviewOverview
Pygo and BCL9/Legless transduce the Wnt signal by promoting the transcriptional activity of beta-catenin/Armadillo in normal and malignant cells. We show that human and Drosophila Pygo PHD fingers associate with their cognate HD1 domains from BCL9/Legless to bind specifically to the histone H3 tail methylated at lysine 4 (H3K4me). The crystal structures of ternary complexes between PHD, HD1, and two different H3K4me peptides reveal a unique mode of histone tail recognition: efficient histone binding requires HD1 association, and the PHD-HD1 complex binds preferentially to H3K4me2 while displaying insensitivity to methylation of H3R2. Therefore, this is a prime example of histone tail binding by a PHD finger (of Pygo) being modulated by a cofactor (BCL9/Legless). Rescue experiments in Drosophila indicate that Wnt signaling outputs depend on histone decoding. The specificity of this process provided by the Pygo-BCL9/Legless complex suggests that this complex facilitates an early step in the transition from gene silence to Wnt-induced transcription.
About this StructureAbout this Structure
2VPD is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex., Fiedler M, Sanchez-Barrena MJ, Nekrasov M, Mieszczanek J, Rybin V, Muller J, Evans P, Bienz M, Mol Cell. 2008 May 23;30(4):507-18. PMID:18498752 Page seeded by OCA on Wed Jun 18 12:05:27 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Homo sapiens
- Protein complex
- Bienz, M.
- Evans, P.
- Fiedler, M.
- Mieszczanek, J.
- Muller, J.
- Nekrasov, M.
- Rybin, V.
- Sanchez-Barrena, M J.
- Bcl9 hd1 domain
- Chromosomal rearrangement
- Gene regulation
- Histone h3k4me2
- Hpygo1 phd domain
- Metal-binding
- Nucleus
- Phosphoprotein
- Proto-oncogene
- Signaling protein
- Wnt signaling complex
- Wnt signaling pathway
- Zinc
- Zinc-finger