2q9f: Difference between revisions
New page: '''Unreleased structure''' The entry 2q9f is ON HOLD until Jun 12 2009 Authors: White, M.A., Mast, N.V., Johnson, E.F., Stout, C.D., Pikuleva, I.A. Description: Crystal structure of hu... |
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{{STRUCTURE_2q9f| PDB=2q9f | SCENE= }} | |||
'''Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate''' | |||
==Overview== | |||
Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogue cholesterol-3-sulfate (CH-3S) is reported. The 2.6 angstroms structure of CYP46A1-CH-3S was solved using both anion and cation heavy-atom salts. In addition to the native anomalous signal from the haem iron, an NaI anion halide salt derivative and a complementary CsCl alkali-metal cation salt derivative were used. The general implications of the use of halide and alkali-metal quick soaks are discussed. The importance of using isoionic strength buffers, the titration of heavy-atom salts into different ionic species and the role of concentration are considered. It was observed that cation/anion-binding sites will occasionally overlap, which could negatively impact upon mixed RbBr soaks used for multiple anomalous scatterer MAD (MMAD). The use of complementary cation and anion heavy-atom salt derivatives is a convenient and powerful tool for MIR(AS) structure solution. | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | ==About this Structure== | ||
2Q9F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q9F OCA]. | |||
==Reference== | |||
Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453684 18453684] | |||
[[Category: Cholesterol 24-hydroxylase]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Single protein]] | |||
[[Category: Johnson, E F.]] | |||
[[Category: Mast, N V.]] | |||
[[Category: Pikuleva, I A.]] | |||
[[Category: Stout, C D.]] | |||
[[Category: White, M A.]] | |||
[[Category: Cholesterol metabolic enzyme]] | |||
[[Category: Cholesterol-3-sulphate]] | |||
[[Category: Cyp46a1]] | |||
[[Category: Heme]] | |||
[[Category: Monooxygenase]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: P450]] | |||
[[Category: P450 46a1]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:03:06 2008'' | |||
Revision as of 12:03, 18 June 2008
Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate
OverviewOverview
Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogue cholesterol-3-sulfate (CH-3S) is reported. The 2.6 angstroms structure of CYP46A1-CH-3S was solved using both anion and cation heavy-atom salts. In addition to the native anomalous signal from the haem iron, an NaI anion halide salt derivative and a complementary CsCl alkali-metal cation salt derivative were used. The general implications of the use of halide and alkali-metal quick soaks are discussed. The importance of using isoionic strength buffers, the titration of heavy-atom salts into different ionic species and the role of concentration are considered. It was observed that cation/anion-binding sites will occasionally overlap, which could negatively impact upon mixed RbBr soaks used for multiple anomalous scatterer MAD (MMAD). The use of complementary cation and anion heavy-atom salt derivatives is a convenient and powerful tool for MIR(AS) structure solution.
About this StructureAbout this Structure
2Q9F is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:18453684 Page seeded by OCA on Wed Jun 18 12:03:06 2008