2k3f: Difference between revisions
New page: '''Unreleased structure''' The entry 2k3f is ON HOLD Authors: Jonker, H.R.A., Ilin, S., Schwalbe, H., Woehnert, J. Description: Ribosomal protein L11 from Thermotoga maritima ''Page ... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2k3f.jpg|left|200px]] | |||
The | <!-- | ||
The line below this paragraph, containing "STRUCTURE_2k3f", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_2k3f| PDB=2k3f | SCENE= }} | |||
'''Ribosomal protein L11 from Thermotoga maritima''' | |||
==Overview== | |||
L11, a protein of the large ribosomal subunit, binds to a highly conserved domain of 23S rRNA and mediates ribosomal GTPase activity. Its C-terminal domain is the main determinant for rRNA binding, whereas its N-terminal domain plays only a limited role in RNA binding. The N-terminal domain is thought to be involved in interactions with elongation and release factors as well as with the antibiotics thiostrepton and micrococcin. This report presents the NMR solution structure of the full-length L11 protein from the thermophilic eubacterium Thermotoga maritima in its free form. The structure is based on a large number of orientational restraints derived from residual dipolar couplings in addition to conventional NOE-based restraints. The solution structure of L11 demonstrates that, in contrast to many other multidomain RNA-binding proteins, the relative orientation of the two domains is well defined. This is shown both by heteronuclear 15N-relaxation and residual dipolar-coupling data. Comparison of this NMR structure with the X-ray structure of RNA-bound L11, reveals that binding not only induces a rigidification of a flexible loop in the C-terminal domain, but also a sizeable reorientation of the N-terminal domain. The domain orientation in free L11 shows limited similarity to that of ribosome-bound L11 in complex with elongation factor, EF-G. | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | ==About this Structure== | ||
2K3F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K3F OCA]. | |||
==Reference== | |||
Domain reorientation and induced fit upon RNA binding: solution structure and dynamics of ribosomal protein L11 from Thermotoga maritima., Ilin S, Hoskins A, Ohlenschlager O, Jonker HR, Schwalbe H, Wohnert J, Chembiochem. 2005 Sep;6(9):1611-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16094695 16094695] | |||
[[Category: Single protein]] | |||
[[Category: Thermotoga maritima]] | |||
[[Category: Ilin, S.]] | |||
[[Category: Jonker, H R.A.]] | |||
[[Category: Schwalbe, H.]] | |||
[[Category: Woehnert, J.]] | |||
[[Category: L11]] | |||
[[Category: Methylation]] | |||
[[Category: Ribonucleoprotein]] | |||
[[Category: Ribosomal protein]] | |||
[[Category: Rna-binding]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:02:19 2008'' | |||