Rop protein: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

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 == Mutants ==
-Numerous mutations in the loop region of Rop have been produced (deletion of 5 a/a of loop ([[1b6q]]), site-directed mutants in loop, replacement and insertion of glycine residues in loop, restored of heptad pattern in loop region).
+Numerous mutations in the loop region of Rop have been produced:
-A31P appears to be trapped in a molten globule state, the reason being that Pro31, unlike Ala31, is more confor-mationally constrained (the dihedral angles of Ala31 in the wild type molecule are prohibited to Pro) and leads to a folding pathway for a thermodynamically less stable confor-mation.
+*deletion of 5 a/a of loop [[(1qx8)]]
+*site-directed mutants in loop
+*replacement and insertion of glycine residues in loop
+*restored of heptad pattern in loop region)[[(1NKD)]]
+*a single alanine to proline substitution [[(1B6Q)]] (Pro31, unlike Ala31, is more confor-mationally constrained, the dihedral angles of Ala31 in the wild type molecule are prohibited to Pro, and leads to a folding pathway for a thermodynamically less stable conformation.)
+*re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle[[(1YO7)]]
+*ALA2ILE2-6, repacted the hydrophobic core and a new fold [[(1F4N)]]
-*A31P [[1b6q]]
-*RM6
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