2b4z: Difference between revisions
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'''Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.''' | '''Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.''' | ||
==Overview== | |||
Cytochrome c is one of the most studied proteins probably due to its electron-transfer properties in aerobic and anaerobic respiration. Particularly, cytochrome c from bovine heart is a small protein, M(r) 12,230 Da, globular (hydrodynamic diameter of 3.4 nm), soluble in different buffer solutions, and commercially available. Despite being a quite well-studied protein and relatively easy to manipulate from the biochemical and electrochemical viewpoint, its 3D structure has never been published. In this work, the purification, crystallization and 3D structure of one of the cytochrome c isoforms is presented to 1.5 A resolution. It is also shown how the presence of isoforms made both the purification and crystallization steps difficult. Finally, a new approach for protein electrocrystallization and design of biosensors is presented. | |||
==About this Structure== | ==About this Structure== | ||
2B4Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4Z OCA]. | 2B4Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4Z OCA]. | ||
==Reference== | |||
High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor., Mirkin N, Jaconcic J, Stojanoff V, Moreno A, Proteins. 2008 Jan 1;70(1):83-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17634981 17634981] | |||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Moreno, A.]] | [[Category: Moreno, A.]] | ||
[[Category: Stojanoff, V.]] | [[Category: Stojanoff, V.]] | ||
[[Category: Electron transport]] | |||
[[Category: Ferric form]] | [[Category: Ferric form]] | ||
[[Category: Low ionic strength]] | [[Category: Low ionic strength]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:41:41 2008'' | ||