9gaa: Difference between revisions

Revision as of 22:52, 4 May 2008

PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM

OverviewOverview

A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins.

About this StructureAbout this Structure

9GAA is a Single protein structure of sequence from Elizabethkingia meningoseptica. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:10490104 Page seeded by OCA on Sun May 4 22:52:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:9gaa.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 9gaa |SIZE=350|CAPTION= <scene name='initialview01'>9gaa</scene>, resolution 2.1&Aring;
+The line below this paragraph, containing "STRUCTURE_9gaa", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_9gaa|  PDB=9gaa  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB]</span>
-}}
 '''PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''
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 Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10490104 10490104]
 [[Category: Elizabethkingia meningoseptica]]
-[[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]]
 [[Category: Single protein]]
 [[Category: Guo, H C.]]
 [[Category: Xu, Q.]]
-[[Category: autoproteolysis]]
+[[Category: Autoproteolysis]]
-[[Category: glycosylasparaginase]]
+[[Category: Glycosylasparaginase]]
-[[Category: mutant]]
+[[Category: Mutant]]
-[[Category: n-terminal nucleophile hydrolase]]
+[[Category: N-terminal nucleophile hydrolase]]
-[[Category: precursor]]
+[[Category: Precursor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:52:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:46:03 2008''