7cat: Difference between revisions

Revision as of 22:44, 4 May 2008

THE NADPH BINDING SITE ON BEEF LIVER CATALASE

OverviewOverview

Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery.

About this StructureAbout this Structure

7CAT is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839 Page seeded by OCA on Sun May 4 22:44:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:7cat.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 7cat |SIZE=350|CAPTION= <scene name='initialview01'>7cat</scene>, resolution 2.5&Aring;
+The line below this paragraph, containing "STRUCTURE_7cat", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_7cat|  PDB=7cat  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7cat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cat OCA], [http://www.ebi.ac.uk/pdbsum/7cat PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7cat RCSB]</span>
-}}
 '''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-CAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CAT OCA].
+CAT is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CAT OCA].
 ==Reference==
@@ Line 31: / Line 28: @@
 [[Category: Sicignano, A.]]
 [[Category: Tanaka, N.]]
-[[Category: oxidoreductase (h2o2 acceptor)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:44:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:43:52 2008''