6fit: Difference between revisions

Revision as of 22:40, 4 May 2008

FHIT-TRANSITION STATE ANALOG

OverviewOverview

The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.

About this StructureAbout this Structure

6FIT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207 Page seeded by OCA on Sun May 4 22:40:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:6fit.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 6fit |SIZE=350|CAPTION= <scene name='initialview01'>6fit</scene>, resolution 2.6&Aring;
+The line below this paragraph, containing "STRUCTURE_6fit", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AVE:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVE</scene> and <scene name='pdbsite=HNE:HIS+Triad+For+Which+This+Family+Was+Named'>HNE</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AMW:ADENOSINE+MONOTUNGSTATE'>AMW</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bis(5'-adenosyl)-triphosphatase Bis(5'-adenosyl)-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.29 3.6.1.29] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= FHIT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_6fit|  PDB=6fit  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fit OCA], [http://www.ebi.ac.uk/pdbsum/6fit PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=6fit RCSB]</span>
-}}
 '''FHIT-TRANSITION STATE ANALOG'''
@@ Line 23: / Line 20: @@
 ==Reference==
 Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9323207 9323207]
-[[Category: Bis(5'-adenosyl)-triphosphatase]]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 29: / Line 25: @@
 [[Category: Klein, M G.]]
 [[Category: Lima, C D.]]
-[[Category: fhit]]
+[[Category: Fhit]]
-[[Category: fragile histidine triad protein]]
+[[Category: Fragile histidine triad protein]]
-[[Category: histidine triad protein family]]
+[[Category: Histidine triad protein family]]
-[[Category: hit protein family]]
+[[Category: Hit protein family]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: nucleotidyl hydrolase]]
+[[Category: Nucleotidyl hydrolase]]
-[[Category: nucleotidyl transferase]]
+[[Category: Nucleotidyl transferase]]
-[[Category: putative tumor suppressor]]
+[[Category: Putative tumor suppressor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:40:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:42:55 2008''