467d: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:467d.gif|left|200px]] | [[Image:467d.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_467d", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_467d| PDB=467d | SCENE= }} | |||
}} | |||
'''The structure of a decamer forming a four-way junction''' | '''The structure of a decamer forming a four-way junction''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=467D OCA]. | |||
==Reference== | ==Reference== | ||
Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504723 10504723] | Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504723 10504723] | ||
[[Category: Coll, M.]] | [[Category: Coll, M.]] | ||
[[Category: Ortiz-Lombardia, M.]] | [[Category: Ortiz-Lombardia, M.]] | ||
[[Category: | [[Category: Four-way-junction]] | ||
[[Category: | [[Category: G:a mismatch]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:17 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 22:19, 4 May 2008
The structure of a decamer forming a four-way junction
OverviewOverview
DNA recombination is a universal biological event responsible both for the generation of genetic diversity and for the maintenance of genome integrity. A four-way DNA junction, also termed Holliday junction, is the key intermediate in nearly all recombination processes. This junction is the substrate of recombination enzymes that promote branch migration or catalyze its resolution. We have determined the crystal structure of a four-way DNA junction by multiwavelength anomalous diffraction, and refined it to 2.16 A resolution. The structure has two-fold symmetry, with pairwise stacking of the double-helical arms, which form two continuous B-DNA helices that run antiparallel, cross in a right-handed way, and contain two G-A mismatches. The exchanging backbones form a compact structure with strong van der Waals contacts and hydrogen bonds, implying that a conformational change must occur for the junction to branch-migrate or isomerize. At the branch point, two phosphate groups from one helix occupy the major groove of the other one, establishing sequence-specific hydrogen bonds. These interactions, together with different stacking energies and steric hindrances, explain the preference for a particular junction stacked conformer.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:10504723 Page seeded by OCA on Sun May 4 22:19:17 2008