3nos: Difference between revisions

Revision as of 22:08, 4 May 2008

HUMAN ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH ARGININE SUBSTRATE

OverviewOverview

Crystal structures of human endothelial nitric oxide synthase (eNOS) and human inducible NOS (iNOS) catalytic domains were solved in complex with the arginine substrate and an inhibitor S-ethylisothiourea (SEITU), respectively. The small molecules bind in a narrow cleft within the larger active-site cavity containing heme and tetrahydrobiopterin. Both are hydrogen-bonded to a conserved glutamate (eNOS E361, iNOS E377). The active-site residues of iNOS and eNOS are nearly identical. Nevertheless, structural comparisons provide a basis for design of isozyme-selective inhibitors. The high-resolution, refined structures of eNOS (2.4 A resolution) and iNOS (2.25 A resolution) reveal an unexpected structural zinc situated at the intermolecular interface and coordinated by four cysteines, two from each monomer.

About this StructureAbout this Structure

3NOS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation., Fischmann TO, Hruza A, Niu XD, Fossetta JD, Lunn CA, Dolphin E, Prongay AJ, Reichert P, Lundell DJ, Narula SK, Weber PC, Nat Struct Biol. 1999 Mar;6(3):233-42. PMID:10074942 Page seeded by OCA on Sun May 4 22:08:05 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:3nos.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3nos |SIZE=350|CAPTION= <scene name='initialview01'>3nos</scene>, resolution 2.400&Aring;
+The line below this paragraph, containing "STRUCTURE_3nos", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HAR:N-OMEGA-HYDROXY-L-ARGININE'>HAR</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_3nos|  PDB=3nos  |  SCENE=  }}
-|RELATEDENTRY=[[4nos|4NOS]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nos OCA], [http://www.ebi.ac.uk/pdbsum/3nos PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3nos RCSB]</span>
-}}
 '''HUMAN ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH ARGININE SUBSTRATE'''
@@ Line 28: / Line 25: @@
 [[Category: Fischmann, T O.]]
 [[Category: Weber, P C.]]
-[[Category: human]]
+[[Category: Human]]
-[[Category: l-arginine monooxygenase]]
+[[Category: L-arginine monooxygenase]]
-[[Category: nitric oxide]]
+[[Category: Nitric oxide]]
-[[Category: zns4]]
+[[Category: Zns4]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:08:05 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:54 2008''