3fua: Difference between revisions

Revision as of 22:02, 4 May 2008

L-FUCULOSE-1-PHOSPHATE ALDOLASE CRYSTAL FORM K

OverviewOverview

The structure of L-fuculose-1-phosphate aldolase in a cubic crystal form has been determined with and without the inhibitor phosphoglycolohydroxamate at 2.4 and 2.7 angstrom (1 angstrom = 0.1 nm) resolution, respectively. This inhibitor mimics the enediolate transition state of the substrate moiety dihydroxyacetone phosphate. The structures showed that dihydroxyacetone phosphate ligates the zinc ion of this metal-dependent class II aldolase with its hydroxyl and keto oxygen atoms, shifting Glu73 away from the zinc coordination sphere to a non-polar environment. At this position Glu73 accepts a proton in the initial reaction step, producing the enediolate which is then stabilized by the zinc ion. The other substrate moiety L-lactaldehyde was modeled, because no binding structure is yet available.

About this StructureAbout this Structure

3FUA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Catalytic mechanism of the metal-dependent fuculose aldolase from Escherichia coli as derived from the structure., Dreyer MK, Schulz GE, J Mol Biol. 1996 Jun 14;259(3):458-66. PMID:8676381 Page seeded by OCA on Sun May 4 22:02:04 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:3fua.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3fua |SIZE=350|CAPTION= <scene name='initialview01'>3fua</scene>, resolution 2.67&Aring;
+The line below this paragraph, containing "STRUCTURE_3fua", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ACT:The+Active+Center+Is+Defined+By+The+Zn+Ion,+The+Four+Zn+...'>ACT</scene> and <scene name='pdbsite=PBS:The+Substrate+Phosphate+Binding+Site+Near+The+Active+Cen+...'>PBS</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_3fua|  PDB=3fua  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fua OCA], [http://www.ebi.ac.uk/pdbsum/3fua PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3fua RCSB]</span>
-}}
 '''L-FUCULOSE-1-PHOSPHATE ALDOLASE CRYSTAL FORM K'''
@@ Line 28: / Line 25: @@
 [[Category: Dreyer, M K.]]
 [[Category: Schulz, G E.]]
-[[Category: class ii aldolase]]
+[[Category: Class ii aldolase]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: zinc enzyme]]
+[[Category: Zinc enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:02:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:33:32 2008''