3boj: Difference between revisions

Revision as of 20:57, 4 May 2008

Carbonic anhydrase from marine diatom Thalassiosira weissflogii- cadmium bound domain 1 without bound metal (CDCA1-R1)

OverviewOverview

Carbonic anhydrase, a zinc enzyme found in organisms from all kingdoms, catalyses the reversible hydration of carbon dioxide and is used for inorganic carbon acquisition by phytoplankton. In the oceans, where zinc is nearly depleted, diatoms use cadmium as a catalytic metal atom in cadmium carbonic anhydrase (CDCA). Here we report the crystal structures of CDCA in four distinct forms: cadmium-bound, zinc-bound, metal-free and acetate-bound. Despite lack of sequence homology, CDCA is a structural mimic of a functional beta-carbonic anhydrase dimer, with striking similarity in the spatial organization of the active site residues. CDCA readily exchanges cadmium and zinc at its active site--an apparently unique adaptation to oceanic life that is explained by a stable opening of the metal coordinating site in the absence of metal. Given the central role of diatoms in exporting carbon to the deep sea, their use of cadmium in an enzyme critical for carbon acquisition establishes a remarkable link between the global cycles of cadmium and carbon.

About this StructureAbout this Structure

3BOJ is a Single protein structure of sequence from Thalassiosira weissflogii. Full crystallographic information is available from OCA.

ReferenceReference

Structure and metal exchange in the cadmium carbonic anhydrase of marine diatoms., Xu Y, Feng L, Jeffrey PD, Shi Y, Morel FM, Nature. 2008 Mar 6;452(7183):56-61. PMID:18322527 Page seeded by OCA on Sun May 4 20:57:37 2008

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OCA

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 [[Image:3boj.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3boj |SIZE=350|CAPTION= <scene name='initialview01'>3boj</scene>, resolution 1.45&Aring;
+The line below this paragraph, containing "STRUCTURE_3boj", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Residue+A+1001'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= cdca1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=67004 Thalassiosira weissflogii])
+-->
-|DOMAIN=
+{{STRUCTURE_3boj|  PDB=3boj  |  SCENE=  }}
-|RELATEDENTRY=[[3bob|3BOB]], [[3boc|3BOC]], [[3boe|3BOE]], [[3boh|3BOH]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3boj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boj OCA], [http://www.ebi.ac.uk/pdbsum/3boj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3boj RCSB]</span>
-}}
 '''Carbonic anhydrase from marine diatom Thalassiosira weissflogii- cadmium bound domain 1 without bound metal (CDCA1-R1)'''
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 [[Category: Shi, Y.]]
 [[Category: Xu, Y.]]
-[[Category: apo form]]
+[[Category: Apo form]]
-[[Category: carbonic anhydrase]]
+[[Category: Carbonic anhydrase]]
-[[Category: marine diatom]]
+[[Category: Marine diatom]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:57:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:51 2008''