3bju: Difference between revisions

Revision as of 20:51, 4 May 2008

Crystal Structure of tetrameric form of human lysyl-tRNA synthetase

OverviewOverview

In mammals, many aminoacyl-tRNA synthetases are bound together in a multisynthetase complex (MSC) as a reservoir of procytokines and regulation molecules for functions beyond aminoacylation. The alpha(2) homodimeric lysyl-tRNA synthetase (LysRS) is tightly bound in the MSC and, under specific conditions, is secreted to trigger a proinflammatory response. Results by others suggest that alpha(2) LysRS is tightly bound into the core of the MSC with homodimeric beta(2) p38, a scaffolding protein that itself is multifunctional. Not understood is how the two dimeric proteins combine to make a presumptive alpha(2)beta(2) heterotetramer and, in particular, the location of the surfaces on LysRS that would accommodate the p38 interactions. Here we present a 2.3-A crystal structure of a tetrameric form of human LysRS. The relatively loose (as seen in solution) tetramer interface is assembled from two eukaryote-specific sequences, one in the catalytic- and another in the anticodon-binding domain. This same interface is predicted to provide unique determinants for interaction with p38. The analyses suggest how the core of the MSC is assembled and, more generally, that interactions and functions of synthetases can be built and regulated through dynamic protein-protein interfaces. These interfaces are created from small adaptations to what is otherwise a highly conserved (through evolution) polypeptide sequence.

About this StructureAbout this Structure

3BJU is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation., Guo M, Ignatov M, Musier-Forsyth K, Schimmel P, Yang XL, Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2331-6. Epub 2008 Feb 13. PMID:18272479 Page seeded by OCA on Sun May 4 20:51:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:3bju.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3bju |SIZE=350|CAPTION= <scene name='initialview01'>3bju</scene>, resolution 2.31&Aring;
+The line below this paragraph, containing "STRUCTURE_3bju", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Residue+B+606'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Residue+B+607'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Residue+B+608'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Residue+C+606'>AC4</scene>, <scene name='pdbsite=AC5:Ca+Binding+Site+For+Residue+C+607'>AC5</scene>, <scene name='pdbsite=AC6:Ca+Binding+Site+For+Residue+C+608'>AC6</scene>, <scene name='pdbsite=AC7:Ca+Binding+Site+For+Residue+D+606'>AC7</scene>, <scene name='pdbsite=AC8:Ca+Binding+Site+For+Residue+D+607'>AC8</scene>, <scene name='pdbsite=AC9:Ca+Binding+Site+For+Residue+D+608'>AC9</scene>, <scene name='pdbsite=BC1:Ca+Binding+Site+For+Residue+A+606'>BC1</scene>, <scene name='pdbsite=BC2:Ca+Binding+Site+For+Residue+A+607'>BC2</scene>, <scene name='pdbsite=BC3:Ca+Binding+Site+For+Residue+A+608'>BC3</scene>, <scene name='pdbsite=BC4:LYS+Binding+Site+For+Residue+B+601'>BC4</scene>, <scene name='pdbsite=BC5:Atp+Binding+Site+For+Residue+B+603'>BC5</scene>, <scene name='pdbsite=BC6:LYS+Binding+Site+For+Residue+C+601'>BC6</scene>, <scene name='pdbsite=BC7:Atp+Binding+Site+For+Residue+C+603'>BC7</scene>, <scene name='pdbsite=BC8:LYS+Binding+Site+For+Residue+D+601'>BC8</scene>, <scene name='pdbsite=BC9:Atp+Binding+Site+For+Residue+D+603'>BC9</scene>, <scene name='pdbsite=CC1:LYS+Binding+Site+For+Residue+A+601'>CC1</scene> and <scene name='pdbsite=CC2:Atp+Binding+Site+For+Residue+A+603'>CC2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= KARS, KIAA0070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_3bju|  PDB=3bju  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bju OCA], [http://www.ebi.ac.uk/pdbsum/3bju PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bju RCSB]</span>
-}}
 '''Crystal Structure of tetrameric form of human lysyl-tRNA synthetase'''
@@ Line 29: / Line 26: @@
 [[Category: Schimmel, P.]]
 [[Category: Yang, X L.]]
-[[Category: aminoacyl-trna synthetase]]
+[[Category: Aminoacyl-trna synthetase]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: cytoplasm]]
+[[Category: Cytoplasm]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: lysyl-trna]]
+[[Category: Lysyl-trna]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: phosphoprotein]]
+[[Category: Phosphoprotein]]
-[[Category: polymorphism]]
+[[Category: Polymorphism]]
-[[Category: protein biosynthesis]]
+[[Category: Protein biosynthesis]]
-[[Category: trna synthetase]]
+[[Category: Trna synthetase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:51:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:03 2008''