3bci: Difference between revisions

Revision as of 20:38, 4 May 2008

Crystal Structure of Staphylococcus aureus DsbA

OverviewOverview

In Gram-negative bacteria, the introduction of disulfide bonds into folding proteins occurs in the periplasm and is catalyzed by donation of an energetically unstable disulfide from DsbA, which is subsequently re-oxidized through interaction with DsbB. Gram-positive bacteria lack a classic periplasm but nonetheless encode Dsb-like proteins. Staphylococcus aureus encodes just one Dsb protein, a DsbA, and no DsbB. Here we report the crystal structure of S. aureus DsbA (SaDsbA), which incorporates a thioredoxin fold with an inserted helical domain, like its Escherichia coli counterpart EcDsbA, but it lacks the characteristic hydrophobic patch and has a truncated binding groove near the active site. These findings suggest that SaDsbA has a different substrate specificity than EcDsbA. Thermodynamic studies indicate that the oxidized and reduced forms of SaDsbA are energetically equivalent, in contrast to the energetically unstable disulfide form of EcDsbA. Further, the partial complementation of EcDsbA by SaDsbA is independent of EcDsbB and biochemical assays show that SaDsbA does not interact with EcDsbB. The identical stabilities of oxidized and reduced SaDsbA may facilitate direct re-oxidation of the protein by extracellular oxidants, without the need for DsbB.

About this StructureAbout this Structure

3BCI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

Staphylococcus aureus DsbA Does Not Have a Destabilizing Disulfide: A NEW PARADIGM FOR BACTERIAL OXIDATIVE FOLDING., Heras B, Kurz M, Jarrott R, Shouldice SR, Frei P, Robin G, Cemazar M, Thony-Meyer L, Glockshuber R, Martin JL, J Biol Chem. 2008 Feb 15;283(7):4261-71. Epub 2007 Dec 12. PMID:18077463 Page seeded by OCA on Sun May 4 20:38:11 2008

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OCA

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- {{Structure
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-|GENE= AAG41993 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bci OCA], [http://www.ebi.ac.uk/pdbsum/3bci PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bci RCSB]</span>
-}}
 '''Crystal Structure of Staphylococcus aureus DsbA'''
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 [[Category: Martin, J L.]]
 [[Category: Thony-Meyer, L.]]
-[[Category: protein folding]]
+[[Category: Protein folding]]
-[[Category: redox active centre]]
+[[Category: Redox active centre]]
-[[Category: redox protein]]
+[[Category: Redox protein]]
-[[Category: thiol-disulfide oxidoreductase]]
+[[Category: Thiol-disulfide oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:38:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:25:06 2008''