3b7a: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:3b7a.jpg|left|200px]] | [[Image:3b7a.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_3b7a", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_3b7a| PDB=3b7a | SCENE= }} | |||
}} | |||
'''Complex of S52A Substituted Droposphila LUSH protein with Ethanol''' | '''Complex of S52A Substituted Droposphila LUSH protein with Ethanol''' | ||
| Line 27: | Line 24: | ||
[[Category: Jones, D N.M.]] | [[Category: Jones, D N.M.]] | ||
[[Category: Thode, A B.]] | [[Category: Thode, A B.]] | ||
[[Category: | [[Category: Behavior]] | ||
[[Category: | [[Category: Odorant binding protein,alochol binding protein]] | ||
[[Category: | [[Category: Olfaction]] | ||
[[Category: | [[Category: Pheromone response]] | ||
[[Category: | [[Category: Pheromone-binding]] | ||
[[Category: | [[Category: Secreted]] | ||
[[Category: | [[Category: Sensory transduction]] | ||
[[Category: | [[Category: Transport]] | ||
[[Category: | [[Category: Transport protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:28:01 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 20:28, 4 May 2008
Complex of S52A Substituted Droposphila LUSH protein with Ethanol
OverviewOverview
It is now generally accepted that many of the physiological effects of alcohol consumption are a direct result of binding to specific sites in neuronal proteins such as ion channels or other components of neuronal signaling cascades. Binding to these targets generally occurs in water-filled pockets and leads to alterations in protein structure and dynamics. However, the precise interactions required to confer alcohol sensitivity to a particular protein remain undefined. Using information from the previously solved crystal structures of the Drosophila melanogaster protein LUSH in complexes with short-chain alcohols, we have designed and tested the effects of specific amino acid substitutions on alcohol binding. The effects of these substitutions, specifically S52A, T57S, and T57A, were examined using a combination of molecular dynamics, X-ray crystallography, fluorescence spectroscopy, and thermal unfolding. These studies reveal that the binding of ethanol is highly sensitive to small changes in the composition of the alcohol binding site. We find that T57 is the most critical residue for binding alcohols; the T57A substitution completely abolishes binding, while the T57S substitution differentially affects ethanol binding compared to longer-chain alcohols. The additional requirement for a potential hydrogen-bond acceptor at position 52 suggests that both the presence of multiple hydrogen-bonding groups and the identity of the hydrogen-bonding residues are critical for defining an ethanol binding site. These results provide new insights into the detailed chemistry of alcohol's interactions with proteins.
About this StructureAbout this Structure
3B7A is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
ReferenceReference
The role of multiple hydrogen-bonding groups in specific alcohol binding sites in proteins: insights from structural studies of LUSH., Thode AB, Kruse SW, Nix JC, Jones DN, J Mol Biol. 2008 Mar 7;376(5):1360-76. Epub 2008 Jan 5. PMID:18234222 Page seeded by OCA on Sun May 4 20:28:01 2008