2znc: Difference between revisions

Revision as of 20:14, 4 May 2008

MURINE CARBONIC ANHYDRASE IV

OverviewOverview

Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. We have determined the 2.8-angstroms resolution crystal structure of a truncated, soluble form of recombinant murine CAIV. We have also determined the structure of its complex with a drug used for glaucoma therapy, the sulfonamide inhibitor brinzolamide (Azopt). The overall structure of murine CAIV is generally similar to that of human CAIV; however, some local structural differences are found in the active site resulting from amino acid sequence differences in the "130's segment" and the residue-63 loop (these may affect the nearby catalytic proton shuttle, His-64). Similar to human CAIV, the C-terminus of murine CAIV is surrounded by a substantial electropositive surface potential that may stabilize the interaction with the phospholipid membrane. Binding interactions observed for brinzolamide rationalize the generally weaker affinity of inhibitors used in glaucoma therapy toward CAIV compared with CAII.

About this StructureAbout this Structure

2ZNC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination., Stams T, Chen Y, Boriack-Sjodin PA, Hurt JD, Liao J, May JA, Dean T, Laipis P, Silverman DN, Christianson DW, Protein Sci. 1998 Mar;7(3):556-63. PMID:9541386 Page seeded by OCA on Sun May 4 20:14:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2znc.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2znc |SIZE=350|CAPTION= <scene name='initialview01'>2znc</scene>, resolution 2.8&Aring;
+The line below this paragraph, containing "STRUCTURE_2znc", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ZN:Zn+Binding+Site'>ZN</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= MURINE CAIV ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_2znc|  PDB=2znc  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2znc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2znc OCA], [http://www.ebi.ac.uk/pdbsum/2znc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2znc RCSB]</span>
-}}
 '''MURINE CARBONIC ANHYDRASE IV'''
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 [[Category: Christianson, D W.]]
 [[Category: Stams, T.]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: murine]]
+[[Category: Murine]]
-[[Category: zinc]]
+[[Category: Zinc]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:14:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:04 2008''