2ze4: Difference between revisions

Revision as of 20:10, 4 May 2008

Crystal structure of phospholipase D from streptomyces antibioticus

OverviewOverview

Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crystallized in six crystal forms using the hanging-drop vapour-diffusion method. The type III and V crystals belong to monoclinic and hexagonal systems, respectively. All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P212121. Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100 A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3 A resolution. A total of 11295 independent reflections to 3 A resolution have been collected from a type VI crystal using a conventional X-ray source, and its structural analysis is currently being conducted using isomorphous replacement methods.

About this StructureAbout this Structure

2ZE4 is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.

ReferenceReference

Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces antibioticus., Suzuki A, Kakuno K, Iwasaki Y, Yamane T, Yamane T, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):317-9. Epub 1999, Jan 1. PMID:10089437 Page seeded by OCA on Sun May 4 20:10:13 2008

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OCA

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 [[Image:2ze4.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_D Phospholipase D], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.4 3.1.4.4] </span>
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-}}
 '''Crystal structure of phospholipase D from streptomyces antibioticus'''
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 [[Category: Suzuki, A.]]
 [[Category: Yamane, T.]]
-[[Category: alpha-beta-beta-alpha-sandwich]]
+[[Category: Alpha-beta-beta-alpha-sandwich]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: lipid degradation]]
+[[Category: Lipid degradation]]
-[[Category: secreted]]
+[[Category: Secreted]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:10:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:40 2008''