2zbf: Difference between revisions

Revision as of 20:06, 4 May 2008

Calcium pump crystal structure with bound BeF3 and TG in the absence of calcium

OverviewOverview

Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum is the best-studied member of the P-type or E1/E2 type ion transporting ATPases. It has been crystallized in seven different states that cover nearly the entire reaction cycle. Here we describe the structure of this ATPase complexed with phosphate analogs BeF(3)(-) and AlF(4)(-) in the absence of Ca(2+), which correspond to the E2P ground state and E2 approximately P transition state, respectively. The luminal gate is open with BeF(3)(-) and closed with AlF(4)(-). These and the E1 approximately P.ADP analog crystal structures show that a two-step rotation of the cytoplasmic A-domain opens and closes the luminal gate through the movements of the M1-M4 transmembrane helices. There are several conformational switches coupled to the rotation, and the one in the cytoplasmic part of M2 has critical importance. In the second step of rotation, positioning of one water molecule couples the hydrolysis of aspartylphosphate to closing of the gate.

About this StructureAbout this Structure

2ZBF is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

ReferenceReference

How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump., Toyoshima C, Norimatsu Y, Iwasawa S, Tsuda T, Ogawa H, Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19831-6. Epub 2007 Dec 5. PMID:18077416 Page seeded by OCA on Sun May 4 20:06:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2zbf.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2zbf |SIZE=350|CAPTION= <scene name='initialview01'>2zbf</scene>, resolution 2.40&Aring;
+The line below this paragraph, containing "STRUCTURE_2zbf", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TG1:OCTANOIC+ACID+[3S-[3ALPHA,+3ABETA,+4ALPHA,+6BETA,+6ABETA,+7BETA,+8ALPHA(Z),+9BALPHA]]-6-(ACETYLOXY)-2,3,-3A,4,5,6,6A,7,8,9B-DECAHYDRO-3,3A-DIHYDROXY-3,6,9-TRIMETHYL-8-[(2-METHYL-1-OXO-2-BUTENYL)OXY]-2-OXO-4-(1-OXOBUTOXY)-AZULENO[4,5-B]FURAN-7-YL+ESTER'>TG1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2zbf|  PDB=2zbf  |  SCENE=  }}
-|RELATEDENTRY=[[1su4|1SU4]], [[1iwo|1IWO]], [[1vfp|1VFP]], [[2zbd|2ZBD]], [[1wpg|1WPG]], [[2agv|2AGV]], [[2dqs|2DQS]], [[2ear|2EAR]], [[2eas|2EAS]], [[2eat|2EAT]], [[2eau|2EAU]], [[2zbe|2ZBE]], [[2zbg|2ZBG]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbf OCA], [http://www.ebi.ac.uk/pdbsum/2zbf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zbf RCSB]</span>
-}}
 '''Calcium pump crystal structure with bound BeF3 and TG in the absence of calcium'''
@@ Line 29: / Line 26: @@
 [[Category: Ogawa, H.]]
 [[Category: Toyoshima, C.]]
-[[Category: alternative splicing]]
+[[Category: Alternative splicing]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: calcium]]
+[[Category: Calcium]]
-[[Category: calcium transport]]
+[[Category: Calcium transport]]
-[[Category: endoplasmic reticulum]]
+[[Category: Endoplasmic reticulum]]
-[[Category: had fold]]
+[[Category: Had fold]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: ion transport]]
+[[Category: Ion transport]]
-[[Category: magnesium]]
+[[Category: Magnesium]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: p-type atpase]]
+[[Category: P-type atpase]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: sarcoplasmic reticulum]]
+[[Category: Sarcoplasmic reticulum]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
-[[Category: transport]]
+[[Category: Transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:06:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:08 2008''