2yqc: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2yqc.gif|left|200px]] | [[Image:2yqc.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2yqc", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_2yqc| PDB=2yqc | SCENE= }} | ||
}} | |||
'''Crystal Structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the apo-like form''' | '''Crystal Structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the apo-like form''' | ||
| Line 31: | Line 28: | ||
[[Category: Nishitani, Y.]] | [[Category: Nishitani, Y.]] | ||
[[Category: Nonaka, T.]] | [[Category: Nonaka, T.]] | ||
[[Category: | [[Category: Candida albican]] | ||
[[Category: | [[Category: Crystal structure]] | ||
[[Category: | [[Category: N-acetylglucosamine]] | ||
[[Category: | [[Category: N-acetylglucosamine-1-phosphate]] | ||
[[Category: | [[Category: Pyrophosphorylase]] | ||
[[Category: | [[Category: Uridine-diphospho-n-acetylglucosamine]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:11:46 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 19:11, 4 May 2008
Crystal Structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the apo-like form
OverviewOverview
Uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc) is a precursor of the bacterial and fungal cell wall. It is also used in a component of N-linked glycosylation and the glycosylphosphoinositol anchor of eukaryotic proteins. It is synthesized from N-acetylglucosamine-1-phosphate (GlcNAc-1-P) and uridine-5'-triphosphate (UTP) by UDP-GlcNAc pyrophosphorylase (UAP). This is an S(N)2 reaction; the non-esterified oxygen atom of the GlcNAc-1-P phosphate group attacks the alpha-phosphate group of UTP. We determined crystal structures of UAP from Candida albicans (CaUAP1) without any ligands and also complexed with its substrate or with its product. The series of structures in different forms shows the induced fit movements of CaUAP1. Three loops approaching the ligand molecule close the active site when ligand is bound. In addition, Lys-421, instead of the metal ion in prokaryotic UAPs, is coordinated by both phosphate groups of UDP-Glc-NAc and acts as a cofactor. However, a magnesium ion enhances the enzymatic activity of CaUAP1, and thus we propose that the magnesium ion increases the affinity between UTP and the enzyme by coordinating to the alpha- and gamma-phosphate group of UTP.
About this StructureAbout this Structure
2YQC is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism., Maruyama D, Nishitani Y, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2007 Jun 8;282(23):17221-30. Epub 2007 Mar 28. PMID:17392279 Page seeded by OCA on Sun May 4 19:11:46 2008