2vic: Difference between revisions

Revision as of 18:51, 4 May 2008

CRYSTAL STRUCTURE OF THE ISHP608 TRANSPOSASE IN COMPLEX WITH LEFT END 26-MER DNA AND MANGANESE

OverviewOverview

The smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis. First, transposon end binding causes a conformational change that aligns catalytically important protein residues within the active site. Subsequent precise cleavage at the left and right ends, the steps that liberate the transposon from its donor site, does not involve a site-specific DNA-binding domain. Rather, cleavage site recognition occurs by complementary base pairing with a TnpA-bound subterminal transposon DNA segment. Thus, the enzyme active site is constructed from elements of both protein and DNA, reminiscent of the interdependence of protein and RNA in the ribosome. Our structural results explain why the transposon ends are asymmetric and how the transposon selects a target site for integration, and they allow us to propose a molecular model for the entire transposition reaction.

About this StructureAbout this Structure

2VIC is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.

ReferenceReference

Mechanism of IS200/IS605 Family DNA Transposases: Activation and Transposon-Directed Target Site Selection., Barabas O, Ronning DR, Guynet C, Hickman AB, Ton-Hoang B, Chandler M, Dyda F, Cell. 2008 Jan 25;132(2):208-20. PMID:18243097 Page seeded by OCA on Sun May 4 18:51:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2vic.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2vic |SIZE=350|CAPTION= <scene name='initialview01'>2vic</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2vic", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+A'>AC1</scene> and <scene name='pdbsite=AC2:Mn+Binding+Site+For+Chain+D'>AC2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2vic|  PDB=2vic  |  SCENE=  }}
-|RELATEDENTRY=[[2a6m|2A6M]], [[2a6o|2A6O]], [[2vju|2VJU]], [[2vjv|2VJV]], [[2vhg|2VHG]], [[2vih|2VIH]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vic OCA], [http://www.ebi.ac.uk/pdbsum/2vic PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vic RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE ISHP608 TRANSPOSASE IN COMPLEX WITH LEFT END 26-MER DNA AND MANGANESE'''
@@ Line 32: / Line 29: @@
 [[Category: Ronning, D R.]]
 [[Category: Ton-Hoang, B.]]
-[[Category: dna stem loop]]
+[[Category: Dna stem loop]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: huh motif]]
+[[Category: Huh motif]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: transposition]]
+[[Category: Transposition]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:51:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:12:40 2008''