2vh5: Difference between revisions

Revision as of 18:48, 4 May 2008

CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX

OverviewOverview

Intracellular antibody fragments that interfere with molecular interactions inside cells are valuable in investigation of interactomes and in therapeutics, but their application demands that they function in the reducing cellular milieu. We show here a 2.7-A crystal structure of intracellular antibody folds based on scaffolds developed from intracellular antibody capture technology, and we reveal that there is no structural or functional difference with or without the intra-domain disulfide bond of the variable domain of heavy chain or the variable domain of light chain. The data indicate that, in the reducing in vivo environment, the absence of the intra-domain disulfide bond is not an impediment to correction of antibody folding or to interaction with antigen. Thus, the structural constraints for in-cell function are intrinsic to variable single-domain framework sequences, providing a generic scaffold for isolation of functional intracellular antibody single domains.

About this StructureAbout this Structure

2VH5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds., Tanaka T, Rabbitts TH, J Mol Biol. 2008 Feb 22;376(3):749-57. Epub 2007 Dec 4. PMID:18187153 Page seeded by OCA on Sun May 4 18:48:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2vh5.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2vh5 |SIZE=350|CAPTION= <scene name='initialview01'>2vh5</scene>, resolution 2.70&Aring;
+The line below this paragraph, containing "STRUCTURE_2vh5", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+R'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+R'>AC2</scene> and <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+H'>AC3</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2vh5|  PDB=2vh5  |  SCENE=  }}
-|RELATEDENTRY=[[121p|121P]], [[1aa9|1AA9]], [[1agp|1AGP]], [[1bkd|1BKD]], [[1crr|1CRR]], [[1ctq|1CTQ]], [[1gnr|1GNR]], [[1iaq|1IAQ]], [[1jai|1JAI]], [[1lf0|1LF0]], [[1nvu|1NVU]], [[1nvv|1NVV]], [[1nvw|1NVW]], [[1nvx|1NVX]], [[1p2s|1P2S]], [[1plk|1PLK]], [[1qra|1QRA]], [[1rvd|1RVD]], [[1wq1|1WQ1]], [[1xcm|1XCM]], [[1xd2|1XD2]], [[1zvq|1ZVQ]], [[1zw6|1ZW6]], [[2c5l|2C5L]], [[2cl6|2CL6]], [[2cl7|2CL7]], [[2clc|2CLC]], [[2cld|2CLD]], [[1clu|1CLU]], [[1crp|1CRP]], [[1crq|1CRQ]], [[1gnp|1GNP]], [[1gnq|1GNQ]], [[1he8|1HE8]], [[1ioz|1IOZ]], [[1jah|1JAH]], [[1k8r|1K8R]], [[1lf5|1LF5]], [[1lfd|1LFD]], [[1p2t|1P2T]], [[1p2u|1P2U]], [[1p2v|1P2V]], [[1pll|1PLL]], [[1q21|1Q21]], [[1xj0|1XJ0]], [[221p|221P]], [[2ce2|2CE2]], [[2cl0|2CL0]], [[2gdp|2GDP]], [[2q21|2Q21]], [[2uzi|2UZI]], [[4q21|4Q21]], [[5p21|5P21]], [[621p|621P]], [[6q21|6Q21]], [[821p|821P]], [[2evw|2EVW]], [[421p|421P]], [[521p|521P]], [[721p|721P]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vh5 OCA], [http://www.ebi.ac.uk/pdbsum/2vh5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vh5 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX'''
@@ Line 28: / Line 25: @@
 [[Category: Tanaka, T.]]
 [[Category: Williams, R L.]]
-[[Category: antibody]]
+[[Category: Antibody]]
-[[Category: cancer therapy]]
+[[Category: Cancer therapy]]
-[[Category: disease mutation]]
+[[Category: Disease mutation]]
-[[Category: golgi apparatus]]
+[[Category: Golgi apparatus]]
-[[Category: gtp-binding]]
+[[Category: Gtp-binding]]
-[[Category: immunoglobulin domain]]
+[[Category: Immunoglobulin domain]]
-[[Category: intrabody]]
+[[Category: Intrabody]]
-[[Category: lipoprotein]]
+[[Category: Lipoprotein]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: methylation]]
+[[Category: Methylation]]
-[[Category: nucleotide- binding]]
+[[Category: Nucleotide- binding]]
-[[Category: nucleotide-binding,immune system]]
+[[Category: Nucleotide-binding,immune system]]
-[[Category: oncogene]]
+[[Category: Oncogene]]
-[[Category: palmitate]]
+[[Category: Palmitate]]
-[[Category: prenylation]]
+[[Category: Prenylation]]
-[[Category: proto-oncogene]]
+[[Category: Proto-oncogene]]
-[[Category: signal transduction]]
+[[Category: Signal transduction]]
-[[Category: signaling protein/immune system]]
+[[Category: Signaling protein/immune system]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:48:14 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:12:13 2008''