2v9p: Difference between revisions

Revision as of 18:26, 4 May 2008

CRYSTAL STRUCTURE OF PAPILLOMAVIRUS E1 HEXAMERIC HELICASE DNA-FREE FORM

OverviewOverview

Concerted, stochastic and sequential mechanisms of action have been proposed for different hexameric AAA+ molecular motors. Here we report the crystal structure of the E1 helicase from bovine papillomavirus, where asymmetric assembly is for the first time observed in the absence of nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt specific conformations linked to positional changes in the DNA-binding hairpins, which follow a wave-like trajectory, as observed previously in the E1/DNA/ADP complex. The protein's assembly thus maintains such an asymmetric state in the absence of DNA and nucleotide cofactors, allowing consideration of the E1 helicase action as the propagation of a conformational wave around the protein ring. The data imply that the wave's propagation within the AAA+ domains is not necessarily coupled with a strictly sequential hydrolysis of ATP. Since a single ATP hydrolysis event would affect the whole hexamer, such events may simply serve to rectify the direction of the wave's motion.

About this StructureAbout this Structure

2V9P is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.

ReferenceReference

Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors., Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA, Nucleic Acids Res. 2007;35(19):6451-7. Epub 2007 Sep 19. PMID:17881379 Page seeded by OCA on Sun May 4 18:26:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2v9p.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2v9p |SIZE=350|CAPTION= <scene name='initialview01'>2v9p</scene>, resolution 3.00&Aring;
+The line below this paragraph, containing "STRUCTURE_2v9p", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Po4+Binding+Site+For+Chain+C'>AC7</scene>, <scene name='pdbsite=AC8:Po4+Binding+Site+For+Chain+C'>AC8</scene>, <scene name='pdbsite=AC9:Mg+Binding+Site+For+Chain+C'>AC9</scene>, <scene name='pdbsite=BC1:Po4+Binding+Site+For+Chain+D'>BC1</scene>, <scene name='pdbsite=BC2:Po4+Binding+Site+For+Chain+E'>BC2</scene>, <scene name='pdbsite=BC3:Po4+Binding+Site+For+Chain+F'>BC3</scene>, <scene name='pdbsite=BC4:Po4+Binding+Site+For+Chain+G'>BC4</scene>, <scene name='pdbsite=BC5:Po4+Binding+Site+For+Chain+G'>BC5</scene>, <scene name='pdbsite=BC6:Mg+Binding+Site+For+Chain+G'>BC6</scene>, <scene name='pdbsite=BC7:Po4+Binding+Site+For+Chain+H'>BC7</scene>, <scene name='pdbsite=BC8:Po4+Binding+Site+For+Chain+H'>BC8</scene>, <scene name='pdbsite=BC9:Mg+Binding+Site+For+Chain+H'>BC9</scene>, <scene name='pdbsite=CC1:Po4+Binding+Site+For+Chain+I'>CC1</scene>, <scene name='pdbsite=CC2:Po4+Binding+Site+For+Chain+I'>CC2</scene>, <scene name='pdbsite=CC3:Mg+Binding+Site+For+Chain+I'>CC3</scene>, <scene name='pdbsite=CC4:Po4+Binding+Site+For+Chain+J'>CC4</scene>, <scene name='pdbsite=CC5:Po4+Binding+Site+For+Chain+K'>CC5</scene> and <scene name='pdbsite=CC6:Po4+Binding+Site+For+Chain+L'>CC6</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2v9p|  PDB=2v9p  |  SCENE=  }}
-|RELATEDENTRY=[[1f08|1F08]], [[1ksy|1KSY]], [[1ksx|1KSX]], [[2gxa|2GXA]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9p OCA], [http://www.ebi.ac.uk/pdbsum/2v9p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v9p RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF PAPILLOMAVIRUS E1 HEXAMERIC HELICASE DNA-FREE FORM'''
@@ Line 31: / Line 28: @@
 [[Category: Sanders, C M.]]
 [[Category: Sizov, D.]]
-[[Category: aaa+ molecular motor]]
+[[Category: Aaa+ molecular motor]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: atpase]]
+[[Category: Atpase]]
-[[Category: dna replication]]
+[[Category: Dna replication]]
-[[Category: dna translocation]]
+[[Category: Dna translocation]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: early protein]]
+[[Category: Early protein]]
-[[Category: helicase]]
+[[Category: Helicase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: nucleus]]
+[[Category: Nucleus]]
-[[Category: replication]]
+[[Category: Replication]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:26:44 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:09:59 2008''