2v74: Difference between revisions

Revision as of 18:18, 4 May 2008

CRYSTAL STRUCTURE OF COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE 1 (CARM1), IN COMPLEX WITH S-ADENOSYL-HOMOCYSTEINE

OverviewOverview

Coactivator-associated arginine methyltransferase (CARM1) is a transcriptional coactivator that methylates Arg17 and Arg26 in histone H3. CARM1 contains a conserved protein arginine methyltransferase (PRMT) catalytic core flanked by unique pre- and post-core regions. The crystal structures of the CARM1 catalytic core in the apo and holo states reveal cofactor-dependent formation of a substrate-binding groove providing a specific access channel for arginine to the active site. The groove is supported by the first eight residues of the post-core region (C-extension), not present in other PRMTs. In vitro methylation assays show that the C-extension is essential for all histone H3 methylation activity, whereas the pre-core region is required for methylation of Arg26, but not Arg17. Kinetic analysis shows Arg17 methylation is potentiated by pre-acetylation of Lys18, and this is reflected in k(cat) rather than K(m). Together with the absence of specificity subsites in the structure, this suggests an electrostatic sensing mechanism for communicating the modification status of vicinal residues as part of the syntax of the 'histone code.'

About this StructureAbout this Structure

2V74 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Insights into histone code syntax from structural and biochemical studies of CARM1 methyltransferase., Yue WW, Hassler M, Roe SM, Thompson-Vale V, Pearl LH, EMBO J. 2007 Oct 17;26(20):4402-12. Epub 2007 Sep 20. PMID:17882261 Page seeded by OCA on Sun May 4 18:18:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2v74.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2v74 |SIZE=350|CAPTION= <scene name='initialview01'>2v74</scene>, resolution 2.70&Aring;
+The line below this paragraph, containing "STRUCTURE_2v74", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Sah+Binding+Site+For+Chain+H'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-arginine_N-methyltransferase Histone-arginine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.125 2.1.1.125] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2v74|  PDB=2v74  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v74 OCA], [http://www.ebi.ac.uk/pdbsum/2v74 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v74 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE 1 (CARM1), IN COMPLEX WITH S-ADENOSYL-HOMOCYSTEINE'''
@@ Line 31: / Line 28: @@
 [[Category: Thompson-Vale, V.]]
 [[Category: Yue, W W.]]
-[[Category: alternative splicing]]
+[[Category: Alternative splicing]]
-[[Category: arginine methyltransferase]]
+[[Category: Arginine methyltransferase]]
-[[Category: chromatin regulator]]
+[[Category: Chromatin regulator]]
-[[Category: co- activator]]
+[[Category: Co- activator]]
-[[Category: cytoplasm]]
+[[Category: Cytoplasm]]
-[[Category: histone modification]]
+[[Category: Histone modification]]
-[[Category: methyltransferase]]
+[[Category: Methyltransferase]]
-[[Category: nucleus]]
+[[Category: Nucleus]]
-[[Category: s-adenosyl-l-methionine]]
+[[Category: S-adenosyl-l-methionine]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:18:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:09:02 2008''