2v6e: Difference between revisions

Revision as of 18:16, 4 May 2008

PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA

OverviewOverview

The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.

About this StructureAbout this Structure

2V6E is a Single protein structure of sequence from Klebsiella phage phiko2. Full crystallographic information is available from OCA.

ReferenceReference

An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664 Page seeded by OCA on Sun May 4 18:16:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2v6e.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2v6e |SIZE=350|CAPTION= <scene name='initialview01'>2v6e</scene>, resolution 3.2&Aring;
+The line below this paragraph, containing "STRUCTURE_2v6e", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Vo4+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2v6e|  PDB=2v6e  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [http://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB]</span>
-}}
 '''PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA'''
@@ Line 28: / Line 25: @@
 [[Category: Ellenberger, T.]]
 [[Category: Huang, W M.]]
-[[Category: dna distortion]]
+[[Category: Dna distortion]]
-[[Category: hairpin telomere]]
+[[Category: Hairpin telomere]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: protelomerase]]
+[[Category: Protelomerase]]
-[[Category: resolvase]]
+[[Category: Resolvase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:16:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:08:53 2008''