2uz1: Difference between revisions

Revision as of 17:50, 4 May 2008

1.65 ANGSTROM STRUCTURE OF BENZALDEHYDE LYASE COMPLEXED WITH 2-METHYL-2,4-PENTANEDIOL

OverviewOverview

Benzaldehyde lyase (BAL; EC 4.1.2.38) is a thiamine diphosphate (ThDP) dependent enzyme that catalyses the enantioselective carboligation of two molecules of benzaldehyde to form (R)-benzoin. BAL has hence aroused interest for its potential in the industrial synthesis of optically active benzoins and derivatives. The structure of BAL was previously solved to a resolution of 2.6 A using MAD experiments on a selenomethionine derivative [Mosbacher et al. (2005), FEBS J. 272, 6067-6076]. In this communication of parallel studies, BAL was crystallized in an alternative space group (P2(1)2(1)2(1)) and its structure refined to a resolution of 1.65 A, allowing detailed observation of the water structure, active-site interactions with ThDP and also the electron density for the co-solvent 2-methyl-2,4-pentanediol (MPD) at hydrophobic patches of the enzyme surface.

About this StructureAbout this Structure

2UZ1 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the ThDP-dependent enzyme benzaldehyde lyase refined to 1.65 A resolution., Maraite A, Schmidt T, Ansorge-Schumacher MB, Brzozowski AM, Grogan G, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt, 7):546-8. Epub 2007 Jun 15. PMID:17620706 Page seeded by OCA on Sun May 4 17:50:48 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:2uz1.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2uz1 |SIZE=350|CAPTION= <scene name='initialview01'>2uz1</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_2uz1", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mpd+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzoin_aldolase Benzoin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.38 4.1.2.38] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2uz1|  PDB=2uz1  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uz1 OCA], [http://www.ebi.ac.uk/pdbsum/2uz1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uz1 RCSB]</span>
-}}
 '''1.65 ANGSTROM STRUCTURE OF BENZALDEHYDE LYASE COMPLEXED WITH 2-METHYL-2,4-PENTANEDIOL'''
@@ Line 31: / Line 28: @@
 [[Category: Maraite, A.]]
 [[Category: Schmidt, T.]]
-[[Category: benzaldehyde]]
+[[Category: Benzaldehyde]]
-[[Category: benzoin]]
+[[Category: Benzoin]]
-[[Category: flavoprotein]]
+[[Category: Flavoprotein]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: thiamine diphosphate]]
+[[Category: Thiamine diphosphate]]
-[[Category: thiamine pyrophosphate]]
+[[Category: Thiamine pyrophosphate]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 17:50:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:06:29 2008''