2shp: Difference between revisions

Revision as of 17:19, 4 May 2008

TYROSINE PHOSPHATASE SHP-2

OverviewOverview

The structure of the SHP-2 tyrosine phosphatase, determined at 2.0 angstroms resolution, shows how its catalytic activity is regulated by its two SH2 domains. In the absence of a tyrosine-phosphorylated binding partner, the N-terminal SH2 domain binds the phosphatase domain and directly blocks its active site. This interaction alters the structure of the N-SH2 domain, disrupting its phosphopeptide-binding cleft. Conversely, interaction of the N-SH2 domain with phosphopeptide disrupts its phosphatase recognition surface. Thus, the N-SH2 domain is a conformational switch; it either binds and inhibits the phosphatase, or it binds phosphoproteins and activates the enzyme. Recognition of bisphosphorylated ligands by the tandem SH2 domains is an integral element of this switch; the C-terminal SH2 domain contributes binding energy and specificity, but it does not have a direct role in activation.

About this StructureAbout this Structure

2SHP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the tyrosine phosphatase SHP-2., Hof P, Pluskey S, Dhe-Paganon S, Eck MJ, Shoelson SE, Cell. 1998 Feb 20;92(4):441-50. PMID:9491886 Page seeded by OCA on Sun May 4 17:19:04 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2shp.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2shp |SIZE=350|CAPTION= <scene name='initialview01'>2shp</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_2shp", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CAT:DODECANE-TRIMETHYLAMINE'>CAT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2shp|  PDB=2shp  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2shp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2shp OCA], [http://www.ebi.ac.uk/pdbsum/2shp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2shp RCSB]</span>
-}}
 '''TYROSINE PHOSPHATASE SHP-2'''
@@ Line 31: / Line 28: @@
 [[Category: Pluskey, S.]]
 [[Category: Shoelson, S E.]]
-[[Category: insulin signaling]]
+[[Category: Insulin signaling]]
-[[Category: sh2 protein]]
+[[Category: Sh2 protein]]
-[[Category: tyrosine phosphatase]]
+[[Category: Tyrosine phosphatase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 17:19:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:03:01 2008''