2rgb: Difference between revisions

Revision as of 16:51, 4 May 2008

Crystal structure of H-RasQ61K-GppNHp

OverviewOverview

Transformation efficiencies of Ras mutants at residue 61 range over three orders of magnitude, but the in vitro GTPase activity decreases 10-fold for all mutants. We show that Raf impairs the GTPase activity of RasQ61L, suggesting that the Ras/Raf complex differentially modulates transformation. Our crystal structures show that, in transforming mutants, switch II takes part in a network of hydrophobic interactions burying the nucleotide and precatalytic water molecule. Our results suggest that Y32 and a water molecule bridging it to the gamma-phosphate in the wild-type structure play a role in GTP hydrolysis in lieu of the Arg finger in the absence of GAP. The bridging water molecule is absent in the transforming mutants, contributing to the burying of the nucleotide. We propose a mechanism for intrinsic hydrolysis in Raf-bound Ras and elucidate structural features in the Q61 mutants that correlate with their potency to transform cells.

About this StructureAbout this Structure

2RGB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf., Buhrman G, Wink G, Mattos C, Structure. 2007 Dec;15(12):1618-29. PMID:18073111 Page seeded by OCA on Sun May 4 16:51:42 2008

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OCA

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 [[Image:2rgb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2rgb |SIZE=350|CAPTION= <scene name='initialview01'>2rgb</scene>, resolution 1.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2rgb", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= HRAS, HRAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2rgb|  PDB=2rgb  |  SCENE=  }}
-|RELATEDENTRY=[[2rga|2RGA]], [[2rgc|2RGC]], [[2rgd|2RGD]], [[2rge|2RGE]], [[2rgg|2RGG]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rgb OCA], [http://www.ebi.ac.uk/pdbsum/2rgb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rgb RCSB]</span>
-}}
 '''Crystal structure of H-RasQ61K-GppNHp'''
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 [[Category: Mattos, C.]]
 [[Category: Wink, G.]]
-[[Category: disease mutation]]
+[[Category: Disease mutation]]
-[[Category: golgi apparatus]]
+[[Category: Golgi apparatus]]
-[[Category: gtp-binding]]
+[[Category: Gtp-binding]]
-[[Category: gtpase]]
+[[Category: Gtpase]]
-[[Category: lipoprotein]]
+[[Category: Lipoprotein]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: methylation]]
+[[Category: Methylation]]
-[[Category: molecular switch protein]]
+[[Category: Molecular switch protein]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: oncoprotein]]
+[[Category: Oncoprotein]]
-[[Category: palmitate]]
+[[Category: Palmitate]]
-[[Category: prenylation]]
+[[Category: Prenylation]]
-[[Category: proto-oncogene]]
+[[Category: Proto-oncogene]]
-[[Category: signaling protein]]
+[[Category: Signaling protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:51:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:00:07 2008''