2raj: Difference between revisions

Revision as of 16:33, 4 May 2008

SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9

OverviewOverview

Sorting nexins (SNXs) form a family of proteins known to interact with components in the endosomal system and to regulate various steps of vesicle transport. Sorting nexin 9 (SNX9) is involved in the late stages of clathrin-mediated endocytosis in non-neuronal cells, where together with the GTPase dynamin, it participates in the formation and scission of the vesicle neck. We report here crystal structures of the functional membrane-remodeling unit of SNX9 and show that it efficiently tubulates lipid membranes in vivo and in vitro. Elucidation of the protein superdomain structure, together with mutational analysis and biochemical and cell biological experiments, demonstrated how the SNX9 PX and BAR domains work in concert in targeting and tubulation of phosphoinositide-containing membranes. The study provides insights into the SNX9-induced membrane modulation mechanism.

About this StructureAbout this Structure

2RAJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The PX-BAR membrane-remodeling unit of sorting nexin 9., Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A, EMBO J. 2007 Nov 14;26(22):4788-800. Epub 2007 Oct 18. PMID:17948057 Page seeded by OCA on Sun May 4 16:33:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2raj.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2raj |SIZE=350|CAPTION= <scene name='initialview01'>2raj</scene>, resolution 2.45&Aring;
+The line below this paragraph, containing "STRUCTURE_2raj", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= SNX9, SH3PX1, SH3PXD3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2raj|  PDB=2raj  |  SCENE=  }}
-|RELATEDENTRY=[[2rai|2RAI]], [[2rak|2RAK]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raj OCA], [http://www.ebi.ac.uk/pdbsum/2raj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2raj RCSB]</span>
-}}
 '''SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9'''
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 [[Category: Rak, A.]]
 [[Category: Rasmuson, E.]]
-[[Category: bar domain]]
+[[Category: Bar domain]]
-[[Category: membrane transport]]
+[[Category: Membrane transport]]
-[[Category: px domain]]
+[[Category: Px domain]]
-[[Category: sorting nexin]]
+[[Category: Sorting nexin]]
-[[Category: structural protein]]
+[[Category: Structural protein]]
-[[Category: tubulation]]
+[[Category: Tubulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:33:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:13 2008''