2ra5: Difference between revisions

Revision as of 16:31, 4 May 2008

Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor

OverviewOverview

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

About this StructureAbout this Structure

2RA5 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

ReferenceReference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461 Page seeded by OCA on Sun May 4 16:31:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2ra5.jpg|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_2ra5", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene>
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-|GENE= SCO6256 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam07702 UTRA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2188 PhnF], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=smart00345 HTH_GNTR]</span>
+{{STRUCTURE_2ra5|  PDB=2ra5  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ra5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra5 OCA], [http://www.ebi.ac.uk/pdbsum/2ra5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ra5 RCSB]</span>
-}}
 '''Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor'''
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 [[Category: Xu, X.]]
 [[Category: Zheng, H.]]
-[[Category: beta structure]]
+[[Category: Beta structure]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: mcsg]]
+[[Category: Mcsg]]
-[[Category: midwest center for structural genomic]]
+[[Category: Midwest center for structural genomic]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi-2]]
+[[Category: Psi-2]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
-[[Category: utra domain]]
+[[Category: Utra domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:31:14 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:05 2008''