2r29: Difference between revisions

Revision as of 16:06, 4 May 2008

Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography

OverviewOverview

The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells. A crystal structure of its antigen binding fragment (Fab) complexed with domain III of the viral envelope glycoprotein, E, showed that the epitope would be partially occluded in the known structure of the mature dengue virus. Nevertheless, antibody could bind to the virus at 37 degrees C, suggesting that the virus is in dynamic motion making hidden epitopes briefly available. A cryo-electron microscope image reconstruction of the virus:Fab complex showed large changes in the organization of the E protein that exposed the epitopes on two of the three E molecules in each of the 60 icosahedral asymmetric units of the virus. The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells.

About this StructureAbout this Structure

2R29 is a Protein complex structure of sequences from Dengue virus 2 and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins., Lok SM, Kostyuchenko V, Nybakken GE, Holdaway HA, Battisti AJ, Sukupolvi-Petty S, Sedlak D, Fremont DH, Chipman PR, Roehrig JT, Diamond MS, Kuhn RJ, Rossmann MG, Nat Struct Mol Biol. 2008 Mar;15(3):312-7. Epub 2008 Feb 10. PMID:18264114 Page seeded by OCA on Sun May 4 16:06:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2r29.jpg|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_2r29", creates the "Structure Box" on the page.
-|SITE=
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-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE= E protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11060 Dengue virus 2])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02832 Flavi_glycop_C], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00099 IGv], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00098 IGc], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam07686 V-set]</span>
+{{STRUCTURE_2r29|  PDB=2r29  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r29 OCA], [http://www.ebi.ac.uk/pdbsum/2r29 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r29 RCSB]</span>
-}}
 '''Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography'''
@@ Line 39: / Line 36: @@
 [[Category: Sedlak,D.]]
 [[Category: Sukupolvi-petty,S.]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: capsid protein]]
+[[Category: Capsid protein]]
-[[Category: cleavage on pair of basic residue]]
+[[Category: Cleavage on pair of basic residue]]
-[[Category: endoplasmic reticulum]]
+[[Category: Endoplasmic reticulum]]
-[[Category: envelope protein]]
+[[Category: Envelope protein]]
-[[Category: fab-antigen complex]]
+[[Category: Fab-antigen complex]]
-[[Category: glycoprotein]]
+[[Category: Glycoprotein]]
-[[Category: helicase]]
+[[Category: Helicase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: multifunctional enzyme]]
+[[Category: Multifunctional enzyme]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: nucleotidyltransferase]]
+[[Category: Nucleotidyltransferase]]
-[[Category: nucleus]]
+[[Category: Nucleus]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: protease]]
+[[Category: Protease]]
-[[Category: ribonucleoprotein]]
+[[Category: Ribonucleoprotein]]
-[[Category: rna replication]]
+[[Category: Rna replication]]
-[[Category: rna-binding]]
+[[Category: Rna-binding]]
-[[Category: rna-directed rna polymerase]]
+[[Category: Rna-directed rna polymerase]]
-[[Category: secreted]]
+[[Category: Secreted]]
-[[Category: serine protease]]
+[[Category: Serine protease]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
-[[Category: transferase]]
+[[Category: Transferase]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
-[[Category: viral nucleoprotein]]
+[[Category: Viral nucleoprotein]]
-[[Category: viral protein/immune system complex]]
+[[Category: Viral protein/immune system complex]]
-[[Category: virion]]
+[[Category: Virion]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:06:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:55:45 2008''