2qrs: Difference between revisions
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'''Crystal Structure of a single chain trimer composed of the MHC I heavy chain H-2Kb Y84A, beta-2microglobulin, and ovalbumin-derived peptide.''' | '''Crystal Structure of a single chain trimer composed of the MHC I heavy chain H-2Kb Y84A, beta-2microglobulin, and ovalbumin-derived peptide.''' | ||
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[[Category: Fremont, D H.]] | [[Category: Fremont, D H.]] | ||
[[Category: Mitaksov, V E.]] | [[Category: Mitaksov, V E.]] | ||
[[Category: | [[Category: Glycoprotein]] | ||
[[Category: | [[Category: Immune response]] | ||
[[Category: | [[Category: Immune system]] | ||
[[Category: | [[Category: Membrane]] | ||
[[Category: | [[Category: Mhc i]] | ||
[[Category: | [[Category: Mhc-i]] | ||
[[Category: | [[Category: Ovalbumin]] | ||
[[Category: | [[Category: Single chain]] | ||
[[Category: | [[Category: Transmembrane]] | ||
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Revision as of 15:33, 4 May 2008
Crystal Structure of a single chain trimer composed of the MHC I heavy chain H-2Kb Y84A, beta-2microglobulin, and ovalbumin-derived peptide.
OverviewOverview
MHC class I peptide complexes (pMHC) are routinely used to enumerate T cell populations and are currently being evaluated as vaccines to tumors and specific pathogens. Herein, we describe the structures of three generations of single-chain pMHC progressively designed for the optimal presentation of covalently associated epitopes. Our ultimate design employs a versatile disulfide trap between an invariant MHC residue and a short C-terminal peptide extension. This general strategy is nondisruptive of native pMHC conformation and T cell receptor engagement. Indeed, cell-surface-expressed MHC complexes with disulfide-trapped epitopes are refractory to peptide exchange, suggesting they will make safe and effective vaccines. Furthermore, we find that disulfide-trap stabilized, recombinant pMHC reagents reliably detect polyclonal CD8 T cell populations as proficiently as conventional reagents and are thus well suited to monitor or modulate immune responses during pathogenesis.
About this StructureAbout this Structure
2QRS is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural engineering of pMHC reagents for T cell vaccines and diagnostics., Mitaksov V, Truscott SM, Lybarger L, Connolly JM, Hansen TH, Fremont DH, Chem Biol. 2007 Aug;14(8):909-22. PMID:17719490 Page seeded by OCA on Sun May 4 15:32:59 2008