2qpl: Difference between revisions

Revision as of 15:22, 4 May 2008

Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue

OverviewOverview

The combined use of a rapid virtual screen of a small fragment library together with a single point enzyme assay has been used for the discovery of novel PNP inhibitors. The availability of readily soakable crystals of bovine PNP has allowed the approach to be experimentally validated by determining the crystal structure of one of the inhibitor-PNP complexes. Comparison of the experimentally determined binding mode with that predicted by the virtual screening shows them to be similar. This represents a starting point for the growth of the ligand into a higher affinity inhibitor.

About this StructureAbout this Structure

2QPL is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue., Pereira HM, Berdini V, Cleasby A, Garratt RC, FEBS Lett. 2007 Oct 30;581(26):5082-6. Epub 2007 Oct 2. PMID:17927987 Page seeded by OCA on Sun May 4 15:22:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2qpl.jpg|left|200px]]
- {{Structure
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span>
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-|GENE=
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-|DOMAIN=
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-|RELATEDENTRY=[[1a9p|1a9p]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qpl OCA], [http://www.ebi.ac.uk/pdbsum/2qpl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qpl RCSB]</span>
-}}
 '''Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue'''
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 [[Category: Garratt, R C.]]
 [[Category: Pereira, H M.]]
-[[Category: acetylation]]
+[[Category: Acetylation]]
-[[Category: glycosyltransferase]]
+[[Category: Glycosyltransferase]]
-[[Category: purine nucleoside phosphorylase]]
+[[Category: Purine nucleoside phosphorylase]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 15:22:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:51:53 2008''