2qh4: Difference between revisions

Revision as of 14:57, 4 May 2008

Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop

OverviewOverview

The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661 Page seeded by OCA on Sun May 4 14:57:48 2008

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OCA

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 [[Image:2qh4.gif|left|200px]]
- {{Structure
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-}}
 '''Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop'''
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 ==About this Structure==
-QH4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH4 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH4 OCA].
 ==Reference==
 Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17889661 17889661]
-[[Category: Protein complex]]
 [[Category: Breece, K E.]]
 [[Category: Chim, N.]]
 [[Category: Feigon, J.]]
 [[Category: Theimer, C A.]]
-[[Category: loop]]
+[[Category: Loop]]
-[[Category: rna]]
+[[Category: Rna]]
-[[Category: sheared ga pair]]
+[[Category: Sheared ga pair]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:57:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:49:18 2008''