2qcx: Difference between revisions

Revision as of 14:44, 4 May 2008

Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine

OverviewOverview

TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.

About this StructureAbout this Structure

2QCX is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:18054064 Page seeded by OCA on Sun May 4 14:44:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2qcx.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2qcx |SIZE=350|CAPTION= <scene name='initialview01'>2qcx</scene>, resolution 2.200&Aring;
+The line below this paragraph, containing "STRUCTURE_2qcx", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Pf1+Binding+Site+For+Residue+A+300'>AC1</scene> and <scene name='pdbsite=AC2:Pf1+Binding+Site+For+Residue+B+300'>AC2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PF1:N-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-N-(2-HYDROXYETHYL)FORMAMIDE'>PF1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= tenA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+-->
-|DOMAIN=
+{{STRUCTURE_2qcx|  PDB=2qcx  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qcx OCA], [http://www.ebi.ac.uk/pdbsum/2qcx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qcx RCSB]</span>
-}}
 '''Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine'''
@@ Line 30: / Line 27: @@
 [[Category: Jenkins, A L.]]
 [[Category: Zhang, Y.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: up-down bundle]]
+[[Category: Up-down bundle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:44:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:47:49 2008''