2qbl: Difference between revisions

Revision as of 14:41, 4 May 2008

Crystal structure of ferric G248T cytochrome P450cam

OverviewOverview

Distal pocket water molecules have been widely implicated in the delivery of protons required in O-O bond heterolysis in the P450 reaction cycle. Targeted dehydration of the cytochrome P450cam (CYP101) distal pocket through mutagenesis of a distal pocket glycine to either valine or threonine results in the alteration of spin state equilibria, and has dramatic consequences on the catalytic rate, coupling efficiency, and kinetic solvent isotope effect parameters, highlighting an important role of the active-site hydration level on P450 catalysis. Cryoradiolysis of the mutant CYP101 oxyferrous complexes further indicates a specific perturbation of proton-transfer events required for the transformation of ferric-peroxo to ferric-hydroperoxo states. Finally, crystallography of the 248Val and 248Thr mutants in both the ferric camphor bound resting state and ferric-cyano adducts shows both the alteration of hydrogen-bonding networks and the alteration of heme geometry parameters. Taken together, these results indicate that the distal pocket microenvironment governs the transformation of reactive heme-oxygen intermediates in P450 cytochromes.

About this StructureAbout this Structure

2QBL is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

ReferenceReference

Alteration of P450 distal pocket solvent leads to impaired proton delivery and changes in heme geometry., Makris TM, von Koenig K, Schlichting I, Sligar SG, Biochemistry. 2007 Dec 11;46(49):14129-40. Epub 2007 Nov 15. PMID:18001135 Page seeded by OCA on Sun May 4 14:41:04 2008

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OCA

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 [[Image:2qbl.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2qbl |SIZE=350|CAPTION= <scene name='initialview01'>2qbl</scene>, resolution 1.800&Aring;
+The line below this paragraph, containing "STRUCTURE_2qbl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= camC, cyp101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])
+-->
-|DOMAIN=
+{{STRUCTURE_2qbl|  PDB=2qbl  |  SCENE=  }}
-|RELATEDENTRY=[[2qbm|2QBM]], [[2qbn|2QBN]], [[2qbo|2QBO]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qbl OCA], [http://www.ebi.ac.uk/pdbsum/2qbl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qbl RCSB]</span>
-}}
 '''Crystal structure of ferric G248T cytochrome P450cam'''
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 [[Category: Schlichting, I.]]
 [[Category: Sligar, S D.]]
-[[Category: conserved active site residue]]
+[[Category: Conserved active site residue]]
-[[Category: cyp101,mutant]]
+[[Category: Cyp101,mutant]]
-[[Category: gly248]]
+[[Category: Gly248]]
-[[Category: heme geometry]]
+[[Category: Heme geometry]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:41:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:47:21 2008''