2q8a: Difference between revisions

Revision as of 14:31, 4 May 2008

Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody

OverviewOverview

Identifying functionally critical regions of the malaria antigen AMA1 (apical membrane antigen 1) is necessary to understand the significance of the polymorphisms within this antigen for vaccine development. The crystal structure of AMA1 in complex with the Fab fragment of inhibitory monoclonal antibody 1F9 reveals that 1F9 binds to the AMA1 solvent-exposed hydrophobic trough, confirming its importance. 1F9 uses the heavy and light chain complementarity-determining regions (CDRs) to wrap around the polymorphic loops adjacent to the trough, but uses a ridge of framework residues to bind to the hydrophobic trough. The resulting 1F9-AMA1-combined buried surface of 2,470 A(2) is considerably larger than previously reported Fab-antigen interfaces. Mutations of polymorphic AMA1 residues within the 1F9 epitope disrupt 1F9 binding and dramatically reduce the binding of affinity-purified human antibodies. Moreover, 1F9 binding to AMA1 is competed by naturally acquired human antibodies, confirming that the 1F9 epitope is a frequent target of immunological attack.

About this StructureAbout this Structure

2Q8A is a Single protein structure of sequence from Mus musculus and Plasmodium falciparum. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody., Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Anders RF, Foley M, Batchelor AH, PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:17907804 Page seeded by OCA on Sun May 4 14:31:20 2008

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OCA

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 [[Image:2q8a.jpg|left|200px]]
- {{Structure
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-|GENE= AMA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q8a OCA], [http://www.ebi.ac.uk/pdbsum/2q8a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q8a RCSB]</span>
-}}
 '''Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody'''
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 [[Category: Gupta, A.]]
 [[Category: Murphy, V J.]]
-[[Category: antigen-antibody complex]]
+[[Category: Antigen-antibody complex]]
-[[Category: immune system]]
+[[Category: Immune system]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:31:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:46:06 2008''