2q2k: Difference between revisions
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'''Structure of nucleic-acid binding protein''' | '''Structure of nucleic-acid binding protein''' | ||
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[[Category: Glover, T.]] | [[Category: Glover, T.]] | ||
[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
[[Category: | [[Category: Dna binding protein/dna complex]] | ||
[[Category: | [[Category: Parb]] | ||
[[Category: | [[Category: Partition]] | ||
[[Category: | [[Category: Protein-dna]] | ||
[[Category: | [[Category: Segregation]] | ||
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Revision as of 14:12, 4 May 2008
Structure of nucleic-acid binding protein
OverviewOverview
The stable inheritance of genetic material depends on accurate DNA partition. Plasmids serve as tractable model systems to study DNA segregation because they require only a DNA centromere, a centromere-binding protein and a force-generating ATPase. The centromeres of partition (par) systems typically consist of a tandem arrangement of direct repeats. The best-characterized par system contains a centromere-binding protein called ParR and an ATPase called ParM. In the first step of segregation, multiple ParR proteins interact with the centromere repeats to form a large nucleoprotein complex of unknown structure called the segrosome, which binds ParM filaments. pSK41 ParR binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats to mediate both transcription autoregulation and segregation. Here we report the structure of the pSK41 segrosome revealed in the crystal structure of a ParR-DNA complex. In the crystals, the 20-mer tandem repeats stack pseudo-continuously to generate the full-length centromere with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a continuous protein super-helical array, wrapping the DNA about its positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and subsequent plasmid segregation.
About this StructureAbout this Structure
2Q2K is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
Segrosome structure revealed by a complex of ParR with centromere DNA., Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N, Nature. 2007 Dec 20;450(7173):1268-71. PMID:18097417 Page seeded by OCA on Sun May 4 14:12:42 2008