2pzb: Difference between revisions

Revision as of 14:03, 4 May 2008

NAD+ Synthetase from Bacillus anthracis

OverviewOverview

The crystal structures of NH(3)-dependent NAD+ synthetase from Bacillus anthracis as the apoenzyme (1.9 A), in complex with the natural catalytic products AMP and pyrophosphate (2.4 A) and in complex with the substrate analog adenosine 5'-(alpha,beta-methylene)triphosphate (2.0 A) have been determined. NAD+ synthetase catalyzes the last step in the biosynthesis of the vitally important cofactor NAD+. In comparison to other NAD+ synthetase crystal structures, the C-terminal His-tagged end of the apoenzyme adopts a novel helical conformation, causing significant compensatory changes in the region. The structural accommodations observed in B. anthracis NAD+ synthetase are remarkable in the absence of adverse affects on enzyme activity. They also illustrate a rare example of the influence of a non-native C-terminal His-tag extension on the structure of a native protein. In contrast to the apoenzyme, when AMP and pyrophosphate or adenosine 5'-(alpha,beta-methylene)triphosphate are bound, the C-terminus adopts a conformation that allows ATP binding and overall the structure then resembles other NAD+ synthetase structures. The structures of NAD+ synthetase complexes from B. anthracis are compared with published X-ray crystal structures of the enzyme from B. subtilis, Escherichia coli and Helicobacter pylori. These comparisons support the novel observation that P1 and P2 loop ordering is not a consequence of crystal contacts but rather a consequence of intrinsic intramolecular interactions within the ordered subunit.

About this StructureAbout this Structure

2PZB is a Single protein structure of sequence from Bacillus anthracis. Full crystallographic information is available from OCA.

ReferenceReference

Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:17642516 Page seeded by OCA on Sun May 4 14:03:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2pzb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2pzb |SIZE=350|CAPTION= <scene name='initialview01'>2pzb</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_2pzb", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= nadE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])
+-->
-|DOMAIN=
+{{STRUCTURE_2pzb|  PDB=2pzb  |  SCENE=  }}
-|RELATEDENTRY=[[2pz8|2PZ8]], [[2pza|2PZA]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pzb OCA], [http://www.ebi.ac.uk/pdbsum/2pzb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pzb RCSB]</span>
-}}
 '''NAD+ Synthetase from Bacillus anthracis'''
@@ Line 24: / Line 21: @@
 Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642516 17642516]
 [[Category: Bacillus anthracis]]
-[[Category: NAD(+) synthase]]
 [[Category: Single protein]]
 [[Category: Brouillette, C G.]]
@@ Line 34: / Line 30: @@
 [[Category: Protasevich, I I.]]
 [[Category: Pruett, P S.]]
-[[Category: bacillus anthracis]]
+[[Category: Bacillus anthracis]]
-[[Category: his-tag]]
+[[Category: His-tag]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: nad+ synthetase]]
+[[Category: Nad+ synthetase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:03:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:42:41 2008''