2pxe: Difference between revisions

Revision as of 13:58, 4 May 2008

Variant 4 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle

OverviewOverview

X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.

About this StructureAbout this Structure

2PXE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

A general strategy to solve the phase problem in RNA crystallography., Keel AY, Rambo RP, Batey RT, Kieft JS, Structure. 2007 Jul;15(7):761-72. PMID:17637337 Page seeded by OCA on Sun May 4 13:58:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2pxe.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2pxe |SIZE=350|CAPTION= <scene name='initialview01'>2pxe</scene>, resolution 2.000&Aring;
+The line below this paragraph, containing "STRUCTURE_2pxe", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE+ION'>NCO</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= ffh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2pxe|  PDB=2pxe  |  SCENE=  }}
-|RELATEDENTRY=[[1dul|1DUL]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pxe OCA], [http://www.ebi.ac.uk/pdbsum/2pxe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pxe RCSB]</span>
-}}
 '''Variant 4 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle'''
@@ Line 29: / Line 26: @@
 [[Category: Kieft, J S.]]
 [[Category: Rambo, R P.]]
-[[Category: cation binding]]
+[[Category: Cation binding]]
-[[Category: gu pair]]
+[[Category: Gu pair]]
-[[Category: hexamine]]
+[[Category: Hexamine]]
-[[Category: rna]]
+[[Category: Rna]]
-[[Category: rna phasing]]
+[[Category: Rna phasing]]
-[[Category: signaling protein/rna complex]]
+[[Category: Signaling protein/rna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:58:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:41:57 2008''