2pvr: Difference between revisions

Revision as of 13:52, 4 May 2008

Crystal structure of the catalytic subunit of protein kinase CK2 (C-terminal deletion mutant 1-335) in complex with two sulfate ions

OverviewOverview

CK2alpha is the catalytic subunit of protein kinase CK2 and a member of the CMGC family of eukaryotic protein kinases like the cyclin-dependent kinases, the MAP kinases and glycogen-synthase kinase 3. We present here a 1.6 A resolution crystal structure of a fully active C-terminal deletion mutant of human CK2alpha liganded by two sulfate ions, and we compare this structure systematically with representative structures of related CMGC kinases. The two sulfate anions occupy binding pockets at the activation segment and provide the structural basis of the acidic consensus sequence S/T-D/E-X-D/E that governs substrate recognition by CK2. The anion binding sites are conserved among those CMGC kinases. In most cases they are neutralized by phosphorylation of a neighbouring threonine or tyrosine side-chain, which triggers conformational changes for regulatory purposes. CK2alpha, however, lacks both phosphorylation sites at the activation segment and structural plasticity. Here the anion binding sites are functionally changed from regulation to substrate recognition. These findings underline the exceptional role of CK2alpha as a constitutively active enzyme within a family of strictly controlled protein kinases.

About this StructureAbout this Structure

2PVR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Evolved to be active: sulfate ions define substrate recognition sites of CK2alpha and emphasise its exceptional role within the CMGC family of eukaryotic protein kinases., Niefind K, Yde CW, Ermakova I, Issinger OG, J Mol Biol. 2007 Jul 13;370(3):427-38. Epub 2007 May 5. PMID:17524418 Page seeded by OCA on Sun May 4 13:52:36 2008

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OCA

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 [[Image:2pvr.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2pvr", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
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-|GENE= CSNK2A1, CK2A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1pjk|1PJK]], [[1lp4|1LP4]], [[1day|1DAY]], [[1ymi|1YMI]], [[1jwh|1JWH]], [[1lpu|1LPU]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pvr OCA], [http://www.ebi.ac.uk/pdbsum/2pvr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pvr RCSB]</span>
-}}
 '''Crystal structure of the catalytic subunit of protein kinase CK2 (C-terminal deletion mutant 1-335) in complex with two sulfate ions'''
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 [[Category: Niefind, K.]]
 [[Category: Yde, C W.]]
-[[Category: cmgc kinase]]
+[[Category: Cmgc kinase]]
-[[Category: eukaryotic protein kinase fold]]
+[[Category: Eukaryotic protein kinase fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:52:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:41:23 2008''