2ps5: Difference between revisions

Revision as of 13:42, 4 May 2008

N225D Trichodiene Synthase: Complex With Mg and Pyrophosphate

OverviewOverview

Trichodiene synthase from Fusarium sporotrichioides contains two metal ion-binding motifs required for the cyclization of farnesyl diphosphate: the "aspartate-rich" motif D(100)DXX(D/E) that coordinates to Mg2+A and Mg2+C, and the "NSE/DTE" motif N(225)DXXSXXXE that chelates Mg2+B (boldface indicates metal ion ligands). Here, we report steady-state kinetic parameters, product array analyses, and X-ray crystal structures of trichodiene synthase mutants in which the fungal NSE motif is progressively converted into a plant-like DDXXTXXXE motif, resulting in a degradation in both steady-state kinetic parameters and product specificity. Each catalytically active mutant generates a different distribution of sesquiterpene products, and three newly detected sesquiterpenes are identified. In addition, the kinetic and structural properties of the Y295F mutant of trichodiene synthase were found to be similar to those of the wild-type enzyme, thereby ruling out a proposed role for Y295 in catalysis.

About this StructureAbout this Structure

2PS5 is a Single protein structure of sequence from Fusarium sporotrichioides. Full crystallographic information is available from OCA.

ReferenceReference

Structural and mechanistic analysis of trichodiene synthase using site-directed mutagenesis: probing the catalytic function of tyrosine-295 and the asparagine-225/serine-229/glutamate-233-Mg2+B motif., Vedula LS, Jiang J, Zakharian T, Cane DE, Christianson DW, Arch Biochem Biophys. 2008 Jan 15;469(2):184-94. Epub 2007 Oct 30. PMID:17996718 Page seeded by OCA on Sun May 4 13:42:47 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:2ps5.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2ps5 |SIZE=350|CAPTION= <scene name='initialview01'>2ps5</scene>, resolution 2.1&Aring;
+The line below this paragraph, containing "STRUCTURE_2ps5", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trichodiene_synthase Trichodiene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.6 4.2.3.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= TRI5, TOX 5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 Fusarium sporotrichioides])
+-->
-|DOMAIN=
+{{STRUCTURE_2ps5|  PDB=2ps5  |  SCENE=  }}
-|RELATEDENTRY=[[1jfg|1JFG]], [[1jfa|1JFA]], [[1kiz|1KIZ]], [[1yyq|1YYQ]], [[2ps8|2PS8]], [[2ps4|2PS4]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps5 OCA], [http://www.ebi.ac.uk/pdbsum/2ps5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ps5 RCSB]</span>
-}}
 '''N225D Trichodiene Synthase: Complex With Mg and Pyrophosphate'''
@@ Line 29: / Line 26: @@
 [[Category: Christianson, D W.]]
 [[Category: Vedula, L S.]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: magnesium,ethylene glycol]]
+[[Category: Magnesium,ethylene glycol]]
-[[Category: nse/dte motif]]
+[[Category: Nse/dte motif]]
-[[Category: site-directed mutagenesis]]
+[[Category: Site-directed mutagenesis]]
-[[Category: terpenoid synthase fold]]
+[[Category: Terpenoid synthase fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:42:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:40:02 2008''