2pk0: Difference between revisions

Revision as of 13:16, 4 May 2008

Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution

OverviewOverview

We solved the crystal structure of Streptococcus agalactiae serine/threonine phosphatase (SaSTP) using a combination of single-wavelength anomalous dispersion phasing and molecular replacement. The overall structure resembles that of previously characterized members of the PPM/PP2C STP family. The asymmetric unit contains four monomers and we observed two novel conformations for the flap domain among them. In one of these conformations, the enzyme binds three metal ions, whereas in the other it binds only two. The three-metal ion structure also has the active site arginine in a novel conformation. The switch between the two- and three-metal ion structures appears to be binding of another monomer to the active site of STP, which promotes binding of the third metal ion. This interaction may mimic the binding of a product complex, especially since the motif binding to the active site contains a serine residue aligning remarkably well with the phosphate found in the human STP structure.

About this StructureAbout this Structure

2PK0 is a Single protein structure of sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Streptococcus agalactiae serine/threonine phosphatase. The subdomain conformation is coupled to the binding of a third metal ion., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, FEBS J. 2007 Jun;274(12):3128-37. Epub 2007 May 22. PMID:17521332 Page seeded by OCA on Sun May 4 13:16:26 2008

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OCA

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 [[Image:2pk0.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2pk0 |SIZE=350|CAPTION= <scene name='initialview01'>2pk0</scene>, resolution 2.65&Aring;
+The line below this paragraph, containing "STRUCTURE_2pk0", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= stp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1311 Streptococcus agalactiae])
+-->
-|DOMAIN=
+{{STRUCTURE_2pk0|  PDB=2pk0  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk0 OCA], [http://www.ebi.ac.uk/pdbsum/2pk0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pk0 RCSB]</span>
-}}
 '''Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution'''
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 [[Category: Rantanen, M K.]]
 [[Category: Rubens, C E.]]
-[[Category: phosphatase]]
+[[Category: Phosphatase]]
-[[Category: serine]]
+[[Category: Serine]]
-[[Category: signaling motif]]
+[[Category: Signaling motif]]
-[[Category: streptococcus agalactiae]]
+[[Category: Streptococcus agalactiae]]
-[[Category: threonine]]
+[[Category: Threonine]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:16:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:36:59 2008''