2pbd: Difference between revisions

Revision as of 12:46, 4 May 2008

Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*

OverviewOverview

Cells sustain high rates of actin filament elongation by maintaining a large pool of actin monomers above the critical concentration for polymerization. Profilin-actin complexes constitute the largest fraction of polymerization-competent actin monomers. Filament elongation factors such as Ena/VASP and formin catalyze the transition of profilin-actin from the cellular pool onto the barbed end of growing filaments. The molecular bases of this process are poorly understood. Here we present structural and energetic evidence for two consecutive steps of the elongation mechanism: the recruitment of profilin-actin by the last poly-Pro segment of vasodilator-stimulated phosphoprotein (VASP) and the binding of profilin-actin simultaneously to this poly-Pro and to the G-actin-binding (GAB) domain of VASP. The actin monomer bound at the GAB domain is proposed to be in position to join the barbed end of the growing filament concurrently with the release of profilin.

About this StructureAbout this Structure

2PBD is a Protein complex structure of sequences from Homo sapiens and Oryctolagus cuniculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP., Ferron F, Rebowski G, Lee SH, Dominguez R, EMBO J. 2007 Oct 31;26(21):4597-606. Epub 2007 Oct 4. PMID:17914456 Page seeded by OCA on Sun May 4 12:46:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2pbd.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2pbd |SIZE=350|CAPTION= <scene name='initialview01'>2pbd</scene>, resolution 1.501&Aring;
+The line below this paragraph, containing "STRUCTURE_2pbd", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= PFN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2pbd|  PDB=2pbd  |  SCENE=  }}
-|RELATEDENTRY=[[2pav|2PAV]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pbd OCA], [http://www.ebi.ac.uk/pdbsum/2pbd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pbd RCSB]</span>
-}}
 '''Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*'''
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 [[Category: Ferron, F.]]
 [[Category: Rebowski, G.]]
-[[Category: actin]]
+[[Category: Actin]]
-[[Category: gab domain]]
+[[Category: Gab domain]]
-[[Category: loading poly-pro site]]
+[[Category: Loading poly-pro site]]
-[[Category: poly-proline]]
+[[Category: Poly-proline]]
-[[Category: profilin]]
+[[Category: Profilin]]
-[[Category: structural protein]]
+[[Category: Structural protein]]
-[[Category: ternary complex]]
+[[Category: Ternary complex]]
-[[Category: vasp]]
+[[Category: Vasp]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 12:46:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:33:49 2008''