2p8q: Difference between revisions

Revision as of 12:37, 4 May 2008

Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain

OverviewOverview

The nuclear import of uridine-rich ribonucleoproteins (U snRNPs) is mediated by the transport adaptor snurportin 1 (SNP1). Similar to importin alpha, SNP1 uses an N-terminal importin beta binding (sIBB) domain to recruit the receptor importin beta and gain access to the nucleus. In this paper, we demonstrate the sIBB-domain has a bipartite nature, which contains two distinct binding determinants for importin beta. The first determinant spans residues 25-65, and includes the previously identified alphaIBB-region of homology. The second binding determinant encompasses residues 1-24 and resembles region 1011-1035 of the nucleoporin 153 (Nup153). The two binding determinants synergize within the sIBB-domain to confer a low nanomolar binding affinity for importin beta, (Kd ~2nM) in an interaction that, in vitro, is displaced by RanGppNHp. We propose that in vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of U snRNPs into the nucleus.

About this StructureAbout this Structure

2P8Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:18187419 Page seeded by OCA on Sun May 4 12:36:59 2008

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OCA

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 [[Image:2p8q.jpg|left|200px]]
- {{Structure
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-|GENE= KPNB1, NTF97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-}}
 '''Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain'''
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 [[Category: Cingolani, G.]]
 [[Category: Mitrousis, G.]]
-[[Category: heat repeat]]
+[[Category: Heat repeat]]
-[[Category: ibb-domain]]
+[[Category: Ibb-domain]]
-[[Category: importin]]
+[[Category: Importin]]
-[[Category: karyopherin]]
+[[Category: Karyopherin]]
-[[Category: protein transport]]
+[[Category: Protein transport]]
-[[Category: snurportin]]
+[[Category: Snurportin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 12:36:59 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr  2 11:31:53 2008''