2p6r: Difference between revisions

Revision as of 12:29, 4 May 2008

Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA

OverviewOverview

To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417 Page seeded by OCA on Sun May 4 12:29:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2p6r.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2p6r |SIZE=350|CAPTION= <scene name='initialview01'>2p6r</scene>, resolution 3.000&Aring;
+The line below this paragraph, containing "STRUCTURE_2p6r", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2p6r|  PDB=2p6r  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [http://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB]</span>
-}}
 '''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-P6R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA].
 ==Reference==
 Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417]
-[[Category: Archaeoglobus fulgidus]]
-[[Category: Protein complex]]
 [[Category: Buettner, K.]]
 [[Category: Hopfner, K P.]]
 [[Category: Nehring, S.]]
-[[Category: archaeal helicase]]
+[[Category: Archaeal helicase]]
-[[Category: dna repair,]]
+[[Category: Dna repair]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: sf2 helicase]]
+[[Category: Sf2 helicase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 12:29:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:30:55 2008''